29 Papers
99 Citations
Pu Yan is an academic researcher from Chinese Academy of Tropical Agricultural Sciences. The author has contributed to research in topics: Papaya ringspot virus & Gene. The author has an hindex of 10, co-authored 25 publications.
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Papers
Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning.
TL;DR: The cloning technique known as “Nimble Cloning” uses the restriction enzyme, SfiI, in combination with the T5 exonuclease, to linearize the vector and generate 3′-overhangs simultaneously.
A cassava common mosaic virus vector for virus-induced gene silencing in cassava
Decai Tuo,Peng Zhou,Pu Yan,Hongguang Cui,Yang Liu,Yang Liu,He Wang,He Wang,Xiukun Yang,Xiukun Yang,Wenbin Liao,Di Sun,Di Sun,Xiaoying Li,Wentao Shen +14 more
TL;DR: This work constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM that causes systemic infection with mild symptoms in cassava and modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome.
Helper component-proteinase (HC-Pro) protein of Papaya ringspot virus interacts with papaya calreticulin
TL;DR: It is proposed that the upregulated expression of PaCRT mRNA may be an early defence-related response toPRSV infection in the host plant, and that interaction between PRSV HC-Pro and PaCRt may be involved in plant calcium signalling pathways which could interfere with virus infection or host defence.
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Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning.
TL;DR: To the authors' knowledge, this is the first report of the construction of an infectious full-length cDNA clone of PLDMV and the splicing of intron-containing transcripts following mechanical inoculation.
Cloning and expression analysis of phytoene desaturase and ζ-carotene desaturase genes in Carica papaya
TL;DR: Results indicated that the differential accumulation of carotenoids in red- and yellow-fleshed papaya varieties might be partly explained by the transcriptional level of CpPDS and CpZDS.