Polokoff Mark A
Merck & Co.
14 Papers
156 Citations
Polokoff Mark A is an academic researcher from Merck & Co.. The author has contributed to research in topics: Peptide sequence & Platelet aggregation inhibitor. The author has an hindex of 9, co-authored 14 publications.
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Papers
Echistatin. A potent platelet aggregation inhibitor from the venom of the viper, Echis carinatus.
TL;DR: The purification procedure included gel filtration on Sephadex G-50, cation-exchange chromatography on Mono S, and C18 reverse-phase high pressure liquid chromatography, and NH2-terminal sequence analysis, and reduction of echistatin abolished its inhibitory activity.
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Characterization and platelet inhibitory activity of bitistatin, a potent arginine-glycine-aspartic acid-containing peptide from the venom of the viper Bitis arietans.
TL;DR: In a canine model of in vivo platelet aggregation, bitistatin is an effective antiplatelet agent to inhibit periodic cyclical flow reductions and exhibits reversible effects of ex vivo platelets aggregation as well as on bleeding time.
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Yeast thioltransferase--the active site cysteines display differential reactivity.
TL;DR: The result suggested that Cys26 could readily initiate nucleophilic attack on disulfide substrates at physiological pH, similar to that of mammalian thioltransferase and Escherichia coli glutaredoxin.
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Complete amino acid sequence of yeast thioltransferase (glutaredoxin).
TL;DR: The amino acid sequence of a thiol transferase isolated from Saccharomyces cerevisiae was determined and showed 51% structural similarity to pig liver thioltransferase and 34% to E. coli glutaredoxin.
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High-level expression in Escherichia coli of a chemically synthesized gene for [Leu-28]echistatin
Zhong Ru Can,Jon H. Condra,Robert J. Gould,Robert A. Zivin,Carl D. Bennett,John W. Jacobs,Paul A. Friedman,Polokoff Mark A +7 more
TL;DR: A gene (Ecs) encoding a platelet aggregation inhibitor, echistatin, has been chemically synthesized and high-level expression of the synthetic [Leu-28]Ecs was achieved by its fusion with the E. coli cheY gene in the expression vector.
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