Philippe Pradelles
French Alternative Energies and Atomic Energy Commission
34 Papers
478 Citations
Philippe Pradelles is an academic researcher from French Alternative Energies and Atomic Energy Commission. The author has contributed to research in topics: Immunoassay & Epitope. The author has an hindex of 13, co-authored 34 publications. Previous affiliations of Philippe Pradelles include Centre national de la recherche scientifique.
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Papers
Enzyme immunoassays of adenosine cyclic 3',5'-monophosphate and guanosine cyclic 3',5'-monophosphate using acetylcholinesterase.
TL;DR: The pure tetrameric form of acetylcholinesterase from the electric eel Electrophorus electricus has been covalently coupled to 2'-O-succinyl-cAMP tyrosine methyl ester and 2-suCCinyl-CGMP allowing the determination of cAMP and cGMP.
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[3] Enzyme immunoassays of eicosanoids using acetylcholinesterase
TL;DR: In the early seventies, the need for the development of sensitive and quantitative assay methods for the analysis of the prostanoids became obvious and two major complementary methods were selected by the scientists: gas-chromatography combined with mass spectrometry and radioimmunoassay (RIA) techniques.
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Development and testing of radio and enzyme immunoassays for acidic fibroblast growth factor (aFGF).
Danièle Caruelle,Jacques Grassi,José Courty,Béatrice Groux-Muscatelli,Philippe Pradelles,Denis Barritault,Jean-Pierre Caruelle +6 more
TL;DR: Two immunoassays of aFGF coupled to the tetrameric form of acetylcholinesterase (aFGF-AchE) as tracer were compared and the aF GF content obtained by these methods correlates with the values obtained by biological assay.
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A solid-phase immobilized epitope immunoassay (SPIE-IA) permitting very sensitive and specific measurement of angiotensin II in plasma
Hervé Volland,Philippe Pradelles,Pierre Ronco,Michel Azizi,Dominique Simon,Christophe Créminon,Jacques Grassi +6 more
TL;DR: A new enzyme immunometric assay for angiotensin II (AII) based on SPIE-IA technology (solid-phase immobilized epitope-immunoassay) was developed, using a monoclonal antibody with optimal properties (mAb3 131) selected from a series of 19 anti-AII mAbs.
38
Enzyme immunoassays for leukotrienes C4 and E4 using acetylcholinesterase.
TL;DR: The chapter discusses successfully developing acetylcholinesterase from Electrophorus electricus as a label for various eicosanoids, thereby providing an enzyme immunoassay with sensitivities equal to or superior to those achieved with 125 I radioactive tracers.
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