Philippe Horvath
DuPont
70 Papers
347 Citations
Philippe Horvath is an academic researcher from DuPont. The author has contributed to research in topics: CRISPR & Cas9. The author has an hindex of 33, co-authored 66 publications. Previous affiliations of Philippe Horvath include Danisco & Laval University.
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Papers
CRISPR provides acquired resistance against viruses in prokaryotes
Rodolphe Barrangou,Christophe Fremaux,Hélène Deveau,Melissa Richards,Patrick Boyaval,Sylvain Moineau,Dennis A. Romero,Philippe Horvath +7 more
TL;DR: It is found that, after viral challenge, bacteria integrated new spacers derived from phage genomic sequences, and CRISPR provided resistance against phages, and resistance specificity is determined by spacer-phage sequence similarity.
Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
TL;DR: It is demonstrated that the Cas9–crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system introduces in vitro a double-strand break at a specific site in DNA containing a sequence complementary to crRNA, paving the way for engineering of universal programmable RNA-guided DNA endonucleases.
2.9K
CRISPR/Cas, the Immune System of Bacteria and Archaea
TL;DR: Clustered regularly interspaced short palindromic repeats (CRISPR) form peculiar genetic loci, which provide acquired immunity against viruses and plasmids by targeting nucleic acid in a sequence-specific manner.
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An updated evolutionary classification of CRISPR–Cas systems
Kira S. Makarova,Yuri I. Wolf,Omer S. Alkhnbashi,Fabrizio Costa,Shiraz A. Shah,Sita J. Saunders,Rodolphe Barrangou,Stan J. J. Brouns,Emmanuelle Charpentier,Daniel H. Haft,Philippe Horvath,Sylvain Moineau,Francisco J. M. Mojica,Rebecca M. Terns,Michael P. Terns,Malcolm F. White,Alexander F. Yakunin,Roger A. Garrett,John van der Oost,Rolf Backofen,Eugene V. Koonin +20 more
TL;DR: An approach combining the analysis of signature protein families and features of the architecture of cas loci that unambiguously partitions most CRISPR–cas loci into distinct classes, types and subtypes is presented.
The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA.
Josiane E. Garneau,Marie Ève Dupuis,Manuela Villion,Dennis A. Romero,Rodolphe Barrangou,Patrick Boyaval,Christophe Fremaux,Philippe Horvath,Alfonso H. Magadán,Sylvain Moineau +9 more
TL;DR: In vivo evidence is provided that the Streptococcus thermophilus CRISPR1/Cas system can also naturally acquire spacers from a self-replicating plasmid containing an antibiotic-resistance gene, leading toplasmid loss.