Philippe Goffin
Université catholique de Louvain
26 Papers
6 Citations
Philippe Goffin is an academic researcher from Université catholique de Louvain. The author has contributed to research in topics: Biology & Lactate racemase. The author has an hindex of 13, co-authored 23 publications. Previous affiliations of Philippe Goffin include GlaxoSmithKline & Gembloux Agro-Bio Tech.
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Papers
High-level production of the low-calorie sugar sorbitol by Lactobacillus plantarum through metabolic engineering.
Victor Ladero,Ana Ramos,Anne Wiersma,Philippe Goffin,André Schanck,Michiel Kleerebezem,Jeroen Hugenholtz,Eddy J. Smid,Pascal Hols +8 more
TL;DR: The capacity of Lactobacillus plantarum, a lactic acid bacterium found in many fermented food products and in the gastrointestinal tract of mammals, to produce Sorbitol from fructose-6-phosphate by reverting the sorbitol catabolic pathway in a mutant strain deficient for both l- and d-lactate dehydrogenase activities is investigated.
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Improved adaptation to heat, cold, and solvent tolerance in Lactobacillus plantarum
TL;DR: Small Hsp overproduction alleviated the reduction in growth rate triggered by exposing exponentially growing cells to heat shock and cold shock and led to an enhanced survival in the presence of butanol or ethanol treatment suggesting a potential role of L. plantarum small Hsps in solvent tolerance.
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Lactate Racemization as a Rescue Pathway for Supplying d-Lactate to the Cell Wall Biosynthesis Machinery in Lactobacillus plantarum
Philippe Goffin,Marie Deghorain,Jean-Luc Mainardi,Isabelle Tytgat,Marie-Christine Champomier-Vergès,Michiel Kleerebezem,Pascal Hols +6 more
TL;DR: The cloning of a six-gene operon, which is involved in lactate racemization activity and is positively regulated by l-lactate, is reported, which suggests that L. plantarum is unable to efficiently synthesize peptidoglycan precursors ending in d-alanine and that the cell wall biosynthesis machinery in this species is specifically dedicated to the production of peptid Glycolytic pyruvate precursor endings.
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Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system
Benoît Desguin,Philippe Goffin,Eric Viaene,Michiel Kleerebezem,Vlad Martin-Diaconescu,Michael J. Maroney,Jean-Paul Declercq,Patrice Soumillion,Pascal Hols +8 more
TL;DR: It is shown that the genetic basis of lactate racemization in Lactobacillus plantarum is determined, and that, unexpectedly, the racemase is a nickel-dependent enzyme with a novel α/β fold.
Characterization and Functional Analysis of the poxB Gene, Which Encodes Pyruvate Oxidase in Lactobacillus plantarum
Frédérique Lorquet,Philippe Goffin,Lidia Muscariello,Jean-Bernard Baudry,Victor Ladero,Margherita Sacco,Michiel Kleerebezem,Pascal Hols +7 more
TL;DR: Constitutive overproduction of PoxB in L. plantarum revealed the predominant role of pyruvate oxidase in the control of acetate production under aerobic conditions and revealed that glucose repression of poxB is mediated by CcpA binding to the cre site identified in the promoter region.
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