PTC gene mutations and expression of SHH, PTC, SMO, and GLI-1 in odontogenic keratocysts

PURPOSE To compare various pre-treatments serving as cleaning procedures of dentin on the bond strength of resin composite promoted by a universal adhesive system applied either in the absence or presence of simulated pulpal pressure. MATERIALS AND METHODS Prior to application of the adhesive system (Scotchbond Universal) and resin composite (Filtek Z250), ground dentin surfaces were given one of five pre-treatments either without or with simulated pulpal pressure: 1) no pre-treatment, adhesive system in "self-etch" mode, 2) phosphoric acid etching, adhesive system in "total-etch" mode, 3) polishing with pumice on prophylaxis cup, 4) air abrasion with AIR-FLOW PLUS powder, 5) air abrasion with AIR-FLOW PERIO powder; n = 20/group of pre-treatment. After storage (37°C, 100% humidity, 24 h), micro shear bond strength was measured and data analyzed with parametric ANOVA including Bonferroni-Holm correction for multiple testing followed by Student's t tests (significance level: α = 0.05). RESULTS The ANOVA found type of pre-treatment and simulated pulpal pressure to have no significant effect on dentin bond strength. The explorative post-hoc tests showed a negative effect of simulated pulpal pressure for phosphoric acid etching (adhesive system in "total-etch" mode; p = 0.020), but not for the other four pre-treatments (all p = 1.000). CONCLUSION Air abrasion with powders containing either erythritol and chlorhexidine (AIR-FLOW PLUS) or glycine (AIR-FLOW PERIO) yielded dentin bond strengths similar to no pre-treatment, phosphoric acid etching, or polishing with pumice. Simulated pulpal pressure reduced the bond strength only when the self-etch adhesive system was used in total-etch mode.

/pdf/exposed-dentin-influence-of-cleaning-procedures-and-wmmsopupl2.pdf

Exposed Dentin: Influence of Cleaning Procedures and Simulated Pulpal Pressure on Bond Strength of a Universal Adhesive System.

Comprehensive keratin profiling reveals different histopathogenesis of keratocystic odontogenic tumor and orthokeratinized odontogenic cyst

The aim of the present study was to evaluate the effect of prior microabrasion on the teeth color change and tooth bleaching effectiveness. Eight sound molars were mesio-distally sectioned and the halves were randomly allocated to receive enamel microabrasion or non-abrasion (control) in one of surfaces (buccal or lingual), while the remaining surface received the other treatment. The tooth color on baseline was evaluated by spectrophotometer (CieL*a*b system). After the microabrasion procedure, the tooth color was measured again. Following, the specimens were bleached with 35% hydrogen peroxide for two sessions with one-week interval. The color was re-evaluated 7 days after each section and 30 days after the second session. The effect of enamel microabrasion on color changes was evaluated by paired T-test. Deltas L*, a*, b*, and E were calculated and data submitted to 2-way repeated measure ANOVA followed by Tukey`s test. Paired T-test was also used to assess possible differences on the ultimate color achieved after tooth bleaching. Enamel microabrasion reduced the lightness and increased the redness of specimens. Specimens that received microabrasion presented higher values of ∆L* than control after each bleaching procedure; and higher ∆a* after the 2nd bleaching session. However, the prior enamel microabrasion did not affect the ultimate values of color parameters. Despite enamel microabrasion have modified the tooth color, this procedure did not affect the ultimate results achieved with tooth bleaching using a high-concentrated hydrogen peroxide.

/pdf/impact-of-microabrasion-on-the-effectiveness-of-tooth-1c3qmx83rd.pdf

Impact of Microabrasion on the Effectiveness of Tooth Bleaching.

Aim and objective The purpose of this study was to assess the effect of hydrogen peroxide concentration on the bleaching efficacy and penetration through the tooth structure. Materials and methods One hundred enamel/dentin specimens with cylindrical shape were obtained from bovine incisors. The surfaces were polished and the size standardized. They were divided into five groups (n = 20), following the concentration of hydrogen peroxide in the bleaching gels: 20, 25, 30, 35, and 40% (w/w). The specimens were placed over artificial pulpal chambers containing acetate buffer solution and bleached for 30 minutes (three applications of 10 minutes each). Aliquots of the acetate solution were collected, and the peroxide concentration was measured by an analytic spectrophotometer. The color of the samples was analyzed using a colorimetric spectrophotometer at the baseline and 7 days after the bleaching procedure. The color difference was calculated using the ∆Eab formula. The data were analyzed by one-way ANOVA and Tukey's test (p Results The peroxide concentrations of 20-30% showed smaller bleaching effect than the higher concentrations (p = 0.001). The peroxide penetration was significantly higher (p = 0.001) for the more concentrated gels (35 and 40%). Conclusion The higher peroxide concentrations enhance the bleaching efficacy, but also increased the peroxide penetration through the tooth structure. Clinical significance In-office bleaching gels with higher concentrations of hydrogen peroxide (35 and 40%) present superior whitening efficacy. Nevertheless, they might also intensify the negative biological effects on the pulpal tissue, since they exhibit increased penetration potential. How to cite this article: Torres CRG, Zanatta RF, Godoy MMM, et al. Influence of Bleaching Gel Peroxide Concentration on Color and Penetration through the Tooth Structure. J Contemp Dent Pract 2021;22(5):479-483.

/pdf/influence-of-bleaching-gel-peroxide-concentration-on-color-3n5wc9cx5b.pdf

Influence of Bleaching Gel Peroxide Concentration on Color and Penetration through the Tooth Structure.

This study aimed to evaluate the efficacy of chemical agents to increase the bleaching effectiveness of 10% carbamide peroxide. Two hundred and ninety enamel-dentin discs were prepared from bovine incisors. The color measurement was performed by a spectrophotometer using the CIE L*a*b*system. The groups were divided according to the bleaching treatment: negative control group (NC): without bleaching; positive control group (PC): bleached with 10% carbamide peroxide gel without any chemical activator; Manganese gluconate (MG); Manganese chloride (MC); Ferrous gluconate (FG); Ferric chloride (FC); and Ferrous sulphate (FS). Three different concentrations (MG, MC, FG, FC: 0.01, 0.02 and 0.03% w/w; FS: 0.001, 0.002 and 0.003% w/w) for each agent were tested. The bleaching gel was applied on the specimens for 8 h, after which they were immersed in artificial saliva for 16 h, during 14 days. Color assessments were made after 7 and 14 days. The data were analyzed by repeated measures analysis of variance and Tukey's test (5%). Generally, the test groups were unable to increase the bleaching effect (ΔE) significantly compared to the PC group. Only for ΔL, significant higher values compared to the PC group could be seen after 7 days in groups MG (0.02%), and FS (0.002 and 0.003%). The NC group showed significantly lower values than all tested groups. It was concluded that for home bleaching procedures, the addition of chemical activators did not produce a bleaching result significantly higher than the use of 10% carbamide peroxide without activation, and that the concentration of chemical activators used did not significantly influence the effectiveness of treatment.

Effect of chemical activation of 10% carbamide peroxide gel in tooth bleaching.

Aim: The aim of this study was to investigate the influence of simulated pulpal pressure on efficacy of bleaching gels. Materials and methods: Cylindrical enamel-dentin specimens from bovine teeth (3 mm diameter, enamel and dentin layer each 1 mm thick) were divided into 4 groups, according to the bleaching treatment: negative control (non-bleached), bleached with 10% carbamide peroxide (CP), bleached with 7.5% hydrogen peroxide (HP) and bleached with 35% hydrogen peroxide. Ten percent CP gel was applied for 8 h/day and 7.5% HP for 1 h/day, during 14 days. For 35% HP treatment, two sessions of 45 minutes each were employed. In intermediate periods specimens were stored in artificial saliva. Experimental groups (n = 19) were subdivided according to the simulation of pulpal pressure (25 mm Hg) during bleaching treatment. Initial color measurement and after bleaching treatment were assessed by spectrophotometry, using CIE L*a*b* system. The data were statistically analyzed by ANOVA and Dunnett’s posthoc tests (p 0.05). Conclusion: It was concluded that all bleaching gels showed bleaching efficacy compared to non-bleached group and that the simulated pulpal pressure did not influence the bleaching outcomes of the tested gels. Clinical significance: Although numerous in vitro studies investigating the efficacy of bleaching agents have been performed, they do not properly simulate the pulpal pressure. In order to make these studies closer to clinical conditions, it

/pdf/influence-of-simulated-pulpal-pressure-on-efficacy-of-54jokq22u2.pdf

Influence of simulated pulpal pressure on efficacy of bleaching gels.

I. Doutor em Patologia Bucal e Professor da Disciplina de Cirurgia e Traumatologia Buco-Maxilo-Facial da Faculdade de Odontologia de São José dos Campos – UNESP. II. Professor Doutor da Disciplina de Cirurgia e Traumatologia Buco-Maxilo-Facial da Faculdade de Odontologia de São José dos Campos – UNESP. III. PhD. no programa de Biopatologia Oral na área de Radiologia e Professor Doutor da Disciplina de Cirurgia e Traumatologia Buco-MaxiloFacial da Faculdade de Odontologia de São José dos Campos – UNESP. IV. Cirurgiãdentista e estagiária pela Faculdade de Odontologia de São José dos Campos UNESP, na Disciplina de Cirurgia e Traumatologia Buco-Maxilo-Facial, Departamento de Diagnóstico e Cirurgia. V. Aluna de graduação e monitora pela Faculdade de Odontologia de São José dos Campos UNESP, Disciplina de Cirurgia e Traumatologia Buco-Maxilo-Facial, Departamento de Diagnóstico e Cirurgia. RESUMO O cisto odontogênico ortoqueratinizado (COO) e o tumor odontogênico queratocístico (TOQ) são lesões distintas, com comportamento clínico e características radiográficas semelhantes. Enquanto o COO é classificado como cisto odontogênico, o TOQ, com base na última classificação da OMS em 2005, passou a ser classificado como neoplasia odontogênica. Essa alteração realizada na classificação do TOQ baseou-se em evidências científicas que constataram uma taxa de proliferação celular dessas lesões não compatível com as lesões císticas, fato esse comprovado mediante os dos elevados índices de recidiva encontrados no TOQ. Em função das semelhanças clínicas e radiográficas, a diferenciação histológica dessas lesões torna-se preponderante para o delineamento de um plano de tratamento conservador ou agressivo. Neste trabalho, relata-se um caso de paciente com 28 anos, gênero masculino, leucoderma, com aumento de volume assintomático em mandíbula, na região dos dentes 33, 34 e 35, todos com vitalidade. Os exames de imagem revelaram lesão radiolúcida em parasínfise e corpo mandibular esquerdo. O paciente foi submetido à punção aspirativa, com resultado negativo, e à biópsia incisional. O material coletado foi enviado a um laboratório de anatomopatologia cujo laudo revelou tumor odontogênico queratocístico. Assim sendo, o paciente foi submetido à cirurgia de enucleação com curetagem marginal, evoluindo sem intercorrências no pós-operatório. O material coletado durante a enucleação foi encaminhado ao laboratório de Patologia Bucal da Faculdade de Odontologia de São José dos Campos – UNESP cujo laudo mostrou lesão cística revestida por epitélio ortoqueratinizado compatível com cisto odontogênico ortoqueratinizado, contradizendo o resultado obtido na biópsia incisional. Atualmente, o paciente encontra-se em proservação há 72 meses, sem indícios de recorrência lesional. Descritores: Cisto Odontogênico Ortoqueratinizado; Tumor Odontogênico Queratocístico; Radiologia; Mandíbula; Osso.

Orthokeratinized Odontogenic Cyst x Keratocystic Odontogenic Tumour: The Importance of Differential Histopathogenesis in the Treatment

The aim of this study was to establish a reproducible protocol using the methodology of hyaline zones around the colonies on specific agar plates for phospholipase and proteinase production. This was an in vitro double-blind experiment, in which the dependent variables were the enzymatic activity measurements (Pz) for the production of phospholipase (Pz-ph) and the production of secreted aspartyl proteinases (Pz-sap). Three independent variables give rise to different measurement protocols. All measurements were carried out at two different moments by four examiners (E1, E2, E3, and E4). The minimum sample size was 30 Candida albicans clinical isolates. Specific agar plates for phospholipase and SAPs production were prepared according the literature. The intra-and inter-examiner reproducibility for each protocol was estimated using the Intraclass Correlation Coefficient (ICC) and its confidence interval (95% CI). Based on the results obtained for both phospholipase and SAPs, there appears to be no consensus on the protocol chosen for each particular examiner. Measuring the colonies in triplicate may be the main factor associated with the increase in measurement accuracy and should therefore take precedence over measuring only one colony. When only one examiner is responsible for taking measurements, a standard protocol should be put in place and the statistical calibration of this researcher should be done prior to data collection. However, if two or more researchers are involved in the assessment of agar plates, our results suggest that the protocols using software to undertake plate reading is preferred.

Reliability of the agar based method to assess the production of degradative enzymes in clinical isolates of Candida albicans.

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Papers

Effect of chemical activation of 10% carbamide peroxide gel in tooth bleaching.

Influence of simulated pulpal pressure on efficacy of bleaching gels.

Orthokeratinized Odontogenic Cyst x Keratocystic Odontogenic Tumour: The Importance of Differential Histopathogenesis in the Treatment

Reliability of the agar based method to assess the production of degradative enzymes in clinical isolates of Candida albicans.