Patrick Ovando-Roche
UCL Institute of Ophthalmology
7 Papers
37 Citations
Patrick Ovando-Roche is an academic researcher from UCL Institute of Ophthalmology. The author has contributed to research in topics: Induced pluripotent stem cell & Stem cell. The author has an hindex of 4, co-authored 6 publications. Previous affiliations of Patrick Ovando-Roche include Imperial College London & University College London.
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Papers
Use of bioreactors for culturing human retinal organoids improves photoreceptor yields.
Patrick Ovando-Roche,Emma L. West,Matthew J. Branch,Robert D. Sampson,Milan Fernando,Peter M. G. Munro,Anastasios Georgiadis,Matteo Rizzi,Magdalena Kloc,Arifa Naeem,Joana Ribeiro,Alexander J. Smith,Anai Gonzalez-Cordero,Robin R. Ali +13 more
TL;DR: Analysis revealed that the use of bioreactors results in improved laminar stratification as well as an increase in the yield of photoreceptor cells bearing cilia and nascent outer-segment-like structures.
Assessment of AAV Vector Tropisms for Mouse and Human Pluripotent Stem Cell-Derived RPE and Photoreceptor Cells.
Anai Gonzalez-Cordero,Debbie Goh,Kamil Kruczek,Arifa Naeem,Milan Fernando,Sophia-Martha Kleine Holthaus,Matsuki Takaaki,Samuel J.I. Blackford,Magdalena Kloc,Leticia Agundez,Robert D. Sampson,Shyamanga Borooah,Patrick Ovando-Roche,Manjit S Mehat,Emma L. West,Alexander J. Smith,Rachael A. Pearson,Robin R. Ali +17 more
TL;DR: It is shown that human embryonic stem cell-derived photoreceptors can be used to establish promoter specificity in human cells in vitro and will aid capsid selection and vector design for preclinical evaluation of gene therapy approaches, such as gene editing, that require the use of human cells and tissues.
TRF2-mediated stabilization of hREST4 is critical for the differentiation and maintenance of neural progenitors.
TL;DR: It is demonstrated that TRF2 plays a critical role in antagonizing the repressive function of neuron‐restrictive silencer factor during the neural differentiation of human embryonic stem cells (hESCs) by enhancing the expression of a truncated REST splice isoform the authors term human REST4 (hREST4) due to its similarity to rodent REST4.
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Antioxidant and lipid supplementation improve the development of photoreceptor outer segments in pluripotent stem cell-derived retinal organoids
Emma L. West,Paromita Majunder,Arifa Naeem,Milan Fernando,M. O’Hara-Wright,Emily C. Lanning,Magdalena Kloc,Joana Ribeiro,Patrick Ovando-Roche,Ian O. Shum,Neeraj Jumbu,Robert D. Sampson,Matthew J. Hayes,James W B Bainbridge,Anastasios Georgiadis,Alexander J. Smith,Anai Gonzalez-Cordero,Robin R. Ali +17 more
TL;DR: In this article , the authors determined conditions that would support outer segment development in maturing human pluripotent stem cells (hPSC)-derived photoreceptors, and used these enhanced conditions to generate iPSC-derived retinal organoids from patients with X-linked retinitis pigmentosa.
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Harnessing the Potential of Human Pluripotent Stem Cells and Gene Editing for the Treatment of Retinal Degeneration
Patrick Ovando-Roche,Anastasios Georgiadis,Alexander J. Smith,Rachael A. Pearson,Robin R. Ali +4 more
TL;DR: This brief review will discuss some of the issues that should be taken into account when carrying out disease modelling and gene editing of retinal cells and the use of human induced pluripotent stem cells (iPSCs), and the importance of using isogenic iPSC lines as controls.
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