Patrick Chong
Public Health Agency of Canada
17 Papers
103 Citations
Patrick Chong is an academic researcher from Public Health Agency of Canada. The author has contributed to research in topics: Biology & Acinetobacter baumannii. The author has an hindex of 11, co-authored 17 publications.
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Papers
Evaluation of MALDI-TOF mass spectroscopy methods for determination of Escherichia coli pathotypes.
Clifford G. Clark,Peter Kruczkiewicz,Cai Guan,Stuart McCorrister,Patrick Chong,John L. Wylie,Paul Van Caeseele,Helen Tabor,Phillip Snarr,Matthew W. Gilmour,Eduardo N. Taboada,Garrett Westmacott +11 more
TL;DR: Results confirmed that the Bruker Biotyper methodology that included extraction of proteins from bacterial cells was capable of identifying E. coli isolates from all pathotypes to the species level and, furthermore, thatThe Bruker extraction and MALDI-TOF MS with the evaluation criteria developed in this work was effective for differentiating most pathotypes.
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Comparative Analysis of Outer Membrane Vesicle Isolation Methods With an Escherichia coli tolA Mutant Reveals a Hypervesiculating Phenotype With Outer-Inner Membrane Vesicle Content.
Shelby L. Reimer,Daniel R. Beniac,Shannon L. Hiebert,Timothy F. Booth,Patrick Chong,Garrett Westmacott,George G. Zhanel,Denice C. Bay +7 more
TL;DR: In this paper, two conventional techniques, ultracentrifugation (UC) and ultradiafiltration (UF), are used interchangeably to isolate OMVs, however, there is concern that each technique may inadvertently alter the properties of isolated OMVs during study, and to address this concern, they compared two OMV isolation methods, UC and UF, with respect to final OMV quantities, size distributions and morphologies using a hypervesiculating Escherichia coli K-12 ΔtolA mutant.
Effect of Incubation Temperature on Antibiotic Resistance and Virulence Factors of Acinetobacter baumannii ATCC 17978.
TL;DR: Investigation of the effect of temperature on the clinically relevant phenotypes displayed by A. baumannii at 37°C and 28°C found upregulation of the Csu and iron uptake proteins at 29°C was a key finding for understanding some of the phenotypes shown by the pathogen.
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MALDI-TOF MS detection of carbapenemase activity in clinical isolates of Enterobacteriaceae spp., Pseudomonas aeruginosa, and Acinetobacter baumannii compared against the Carba-NP assay.
Patrick Chong,Stuart McCorrister,Mark S. Unger,David A. Boyd,Michael R. Mulvey,Garrett Westmacott +5 more
TL;DR: MALDI-TOF MS detection of carbapenemase-activity in Gram-negative bacteria was compared against the Carba-NP assay, which could not consistently identify OXA- or GES carbapanemase activity.
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Rapid, Sensitive, and Specific Escherichia coli H Antigen Typing by Matrix-Assisted Laser Desorption Ionization–Time of Flight-Based Peptide Mass Fingerprinting
Huixia Chui,Michael Chan,Drexler Hernandez,Patrick Chong,Stuart McCorrister,Alyssia Robinson,Matthew G. Walker,Lorea A. M. Peterson,Sam Ratnam,David Haldane,Sadjia Bekal,John L. Wylie,Linda Chui,Garrett Westmacott,Bianli Xu,Mike Drebot,Mike Drebot,Celine Nadon,Celine Nadon,J. David Knox,J. David Knox,Gehua Wang,Keding Cheng,Keding Cheng +23 more
TL;DR: A rapid method to identify the Escherichia coli flagellar antigen (H antigen) at the subspecies level was developed using a MALDI-TOF MS platform with high specificity and sensitivity, and DHB (2,5-dihydroxybenzoic acid) worked better than CHCA (α-cyano-4-hydroxycinnamic acid) as the matrix, with higher confidence during protein identification.
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