Paolo Di Simplicio
University of Siena
40 Papers
529 Citations
Paolo Di Simplicio is an academic researcher from University of Siena. The author has contributed to research in topics: Glutathione & Cysteine. The author has an hindex of 20, co-authored 40 publications.
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Papers
In vitro inhibition of human and rat platelets by NO donors, nitrosoglutathione, sodium nitroprusside and SIN-1, through activation of cGMP-independent pathways.
Raffaella Priora,Antonios Margaritis,Simona Frosali,Lucia Coppo,Domenico Summa,Danila Di Giuseppe,Carlo Aldinucci,G. P. Pessina,Anna Di Stefano,Paolo Di Simplicio +9 more
TL;DR: Interestingly, the species difference in NO release from GSNO and inhibition by cGMP-independent mechanism was respectively attributed to S-nitrosylation of non-essential and essential protein SH groups.
Minor thiols cysteine and cysteinylglycine regulate the competition between glutathione and protein SH groups in human platelets subjected to oxidative stress.
Daniela Giustarini,Giuseppe Campoccia,Giuseppe Fanetti,Ranieri Rossi,F. Giannerini,Lorenzo Lusini,Paolo Di Simplicio +6 more
TL;DR: Changes in the concentrations of protein-mixed disulfides (XS-SP) of glutathione (GSH), cysteine (CSH), and cysteinylglycine (CGSH) were studied in human platelets treated with diamide and t-BOOH in timecourse experiments to understand the contribution of minor thiols CSH and CGSH to the regulation of glutATHione-protein mixed disulfide levels in platelets.
Platelet antioxidant enzymes in insulin-dependent diabetes mellitus.
Giuseppe Seghieri,Paolo Di Simplicio,Roberto Anichini,Lorenzo Alviggi,Alessandra De Bellis,Federico Bennardini,Flavia Franconi +6 more
TL;DR: The results suggest that diabetic nephropathy, at least in its early stage, may be related to an altered redox state of platelets, as tested by the reduction in SOD activity, thus, indicating that the renal damage in these patients may be associated to a selective increase in platelet susceptibility to variation in theredox state.
A structurally driven analysis of thiol reactivity in mammalian albumins
Ottavia Spiga,Domenico Summa,Simone Cirri,Andrea Bernini,Vincenzo Venditti,Matteo De Chiara,Raffaella Priora,Simona Frosali,Antonios Margaritis,Danila Di Giuseppe,Paolo Di Simplicio,Neri Niccolai +11 more
TL;DR: Molecular dynamics simulations performed on experimental and predicted albumin structures reveal that thiolation rates are influenced by hydrogen bonding pattern and stability of the acceptor C34 sulphur atom with donor groups of nearby residues.
Blood Glutathione Disulfide: In Vivo Factor or in Vitro Artifact?
Ranieri Rossi,Aldo Milzani,Isabella Dalle-Donne,Daniela Giustarini,Lorenzo Lusini,Roberto Colombo,Paolo Di Simplicio +6 more
TL;DR: GSSG is present in healthy human blood at low concentrations (2-6 micromol/L), and most published data on GSSG may be affected by artifacts.