P. Nolte
5 Papers
7 Citations
P. Nolte is an academic researcher. The author has contributed to research in topics: Gene & DNA methylation. The author has an hindex of 1, co-authored 1 publications.
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Papers
Cell division drives DNA methylation loss in late-replicating domains in primary human cells
TL;DR: In this article , the authors present experimental evidence that DNA hypomethylation is directly driven by proliferation-associated DNA replication within partially methylated domains (PMDs), late-replicating regions of the genome attached to the nuclear lamina.
Temperature Dependence of Thermal and Nonthermal Regulation: Hypothalamic Thermo- and Osmoregulation in the Duck
TL;DR: Temperature dependence of central interneurons was assumed first by Hammel in 1965 to account for the influence of hypothalamic temperature (Thy) on thermoregulatory activities and, consequently, a decrease in gain of input-to-effector coupling with decreasing Thy was assumed.
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High-coverage allele-resolved single-cell DNA methylation profiling reveals cell lineage, X-inactivation state, and replication dynamics
Nathan J. Spix,Walid Abi Habib,Zhouwei Zhang,Emily Eugster,Hsiao-yun Milliron,David Sokol,KwangHo Lee,P. Nolte,Jamie L. Endicott,Kelly F. Krzyzanowski,Toshinori Hinoue,Jacob Morrison,Benjamin K. Johnson,Wanding Zhou,Peter W. Laird +14 more
TL;DR: Researchers develop scDEEP-mC, an improved single-cell whole-genome bisulfite sequencing method, enabling high-coverage allele-resolved DNA methylation profiling, cell lineage identification, and analysis of X-inactivation and replication dynamics in single cells.
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Abstract IA023: Spatial reconstruction and temporal inference of DNA methylation alterations in primary human colorectal cancer
Peter W. Laird,Nathan J. Spix,Hsiao-yun Milliron,Manpreet Kalkat,Emily Eugster,David Sokol,Emily Jung,P. Nolte,Kelly K. Krzyzanowski,Toshinori Hinoue,Hui Shen +10 more
TL;DR: A tumor processing pipeline is developed to deeply and comprehensively map DNA methylation patterns in primary human colorectal cancer (CRC) and adjacent tissues, while preserving spatial information, enabling inference of the temporal order of molecular alterations.
Abstract B033: Mapping subclonal epigenetic evolution in colorectal cancer by spatial analysis of DNA hypermethylation
Nathan J. Spix,Hsiao-yun Milliron,Manpreet Kalkat,Emily Eugster,David W. Chesla,David Sokol,Emily Jung,P. Nolte,Kelly K. Krzyzanowski,Toshinori Hinoue,Hui Shen,Peter W. Laird +11 more
TL;DR: A protocol that allows for the extraction of dozens of small, spatially annotated mini-bulk samples from live tumors to better understand the timing and evolution of PTG hypermethylation events in CRC is developed, suggesting that PTG hypermethylation may be a very early event in carcinogenesis and may play a permissive or even active role in that process.