Oscar P. Kuipers

TL;DR: A microscopy method used in several recent studies is provided, which allows following and recording (fluorescence of) individual bacterial cells of Bacillus subtilis and Streptococcus pneumoniae through growth and division for many generations, and can be used to construct phylogenetic lineage trees by tracing back the history of a single cell within a population that originated from one common ancestor.

TL;DR: In this article, a chemostat modified for culturing an asporogenous B.subtilis sigF mutant strain at extremely low growth rates (also named a retentostat) was set up, and biomass accumulation, culture viability, metabolite production and cell morphology were analysed.

TL;DR: This study shows that although B. subtilis ATCC 6633 and 168 are able to produce various processed lantibiotic peptides, an evident advantage of using either the 8-fold protease-deficient strain WB800 or the genome-minimized B. subilis 168 strain PG10 is the lack of extracellular serine protease activity, which means potential toxicity toward the production host is prevented.

TL;DR: It is shown that a riboswitch generates long-term, stable heterogenous expression of a high-affinity methionine transporter in auxotrophic Lactococcus lactis, and hypothesize that T-box riboswitches, which are commonly found in the Lactobacillales, may play as-yet unexplored roles in the predominantly Auxotrophic lifestyle of these bacteria.