Adherence of bacteria to hydrocarbons: A simple method for measuring cell‐surface hydrophobicity

The ability of bacteria to attach to surfaces and develop into a biofilm has been of considerable interest to many groups in numerous industries, including the medical and food industry. However, little is understood in the critical initial step seen in all biofilm development, the initial bacterial cell attachment to a surface. This initial attachment is critical for the formation of a bacterial biofilm, as all other cells within a biofilm structure rely on the interaction between surface and bacterial cell for their survival. This review examines what are believed to be some of the most important aspects involved in bacterial attachment to a surface.

Bacterial cell attachment, the beginning of a biofilm.

https://mmbr.asm.org/content/mmbr/46/2/129.full.pdf

Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains.

Cell surface charge and hydrophobicity of Bacillus subtilis, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, Serratia marcescens, Staphylococcus aureus, and Staphylococcus epidermidis were determined by hydrocarbon adherence, hydrophobic interaction, and electrostatic interaction chromatography. Surface charge and hydrophobicity were compared with the initial attachment values and rates of attachment of the bacteria to meat surfaces. There was a linear correlation between the relative negative charge on the bacterial cell surface and initial attachment to lean beef muscle (r2 = 0.885) and fat tissue (r2 = 0.777). Hydrophobicity correlated well with attachment to fat tissue only. The relative hydrophobicity of each bacterium was dependent on the specific method of determination, with wide variations noted between methods.

/pdf/cell-surface-charge-characteristics-and-their-relationship-11z28y80en.pdf

Cell surface charge characteristics and their relationship to bacterial attachment to meat surfaces

Microorganisms produce biosurfactant (BS)/bioemulsifier (BE) with wide structural and functional diversity which consequently results in the adoption of different techniques to investigate these diverse amphiphilic molecules. This review aims to compile information on different microbial screening methods, surface active products extraction procedures, and analytical terminologies used in this field. Different methods for screening microbial culture broth or cell biomass for surface active compounds production are also presented and their possible advantages and disadvantages highlighted. In addition, the most common methods for purification, detection, and structure determination for a wide range of BS and BE are introduced. Simple techniques such as precipitation using acetone, ammonium sulphate, solvent extraction, ultrafiltration, ion exchange, dialysis, ultrafiltration, lyophilization, isoelectric focusing (IEF), and thin layer chromatography (TLC) are described. Other more elaborate techniques including high pressure liquid chromatography (HPLC), infra red (IR), gas chromatography-mass spectroscopy (GC-MS), nuclear magnetic resonance (NMR), and fast atom bombardment mass spectroscopy (FAB-MS), protein digestion and amino acid sequencing are also elucidated. Various experimental strategies including static light scattering and hydrodynamic characterization for micelles have been discussed. A combination of various analytical methods are often essential in this area of research and a numbers of trials and errors to isolate, purify and characterize various surface active agents are required. This review introduces the various methodologies that are indispensable for studying biosurfactants and bioemulsifiers.

/pdf/methods-for-investigating-biosurfactants-and-bioemulsifiers-5fq935ybnz.pdf

Methods for investigating biosurfactants and bioemulsifiers: a review

Porcine enteropathogenic Escherichia coli strains possessing or lacking K88 antigen were studied by using hydrophobic interaction chromatography on cross-linked agarose gels with alkyl or aryl substituents (amphiphilic gels) to determine whether or not they possessed surface-associated hydrophobic properties. Strains with K88ab or K88ac antigen adsorbed to phenyl and octyl Sepharose gels in the presence of 4 M sodium chloride. This property correlated with phenotypic expression of K88 antigen. Cells grown at 37 degrees C but not those grown at 18 degrees C possessed hydrophobic adsorptive characteristics in addition to the property of mannose-resistant hemagglutination of guinea pig erythrocytes. Adsorption of K88-positive strains to gels with hydrophobic ligands was independent of O group and enterotoxicity. Strains lacking K88 antigen did not adsorb to the hydrophobically substituted derivatives of Sepharose and lacked mannose-resistant hemagglutinating characteristics. Neither the presence of additional polysaccharide K antigens nor nonhemagglutinating pili conferred the property of hydrophobic interaction on the strains. K88-positive bacteria had a lower electrophoretic migration rate than did K88-negative bacteria of the same serotype in free-zone electrophoresis. K88-positive bacteria also adsorbed strongly to hydrophobic ligands in the presence of 1 M ammonium sulfate, whereas K88-negative strains did not. These observations provide evidence for the suspected role of hydrophobic interaction in the adhesive properties of certain enteropathogenic strains of E. coli. Moreover, hydrophobic interaction chromatography provides convenient and rapid alternative means of screening strains for a property potentially associated with adhesiveness.

https://iai.asm.org/content/iai/22/2/462.full.pdf

Differences in hydrophobic surface characteristics of porcine enteropathogenic Escherichia coli with or without K88 antigen as revealed by hydrophobic interaction chromatography.

To evaluate the effect of widely used parenteral vaccination of dams against neonatal colibacillosis, the virulence factors of the intestinal Escherichia coli flora, namely, O serogroup, enterotoxin(s) produced (heat labile, porcine heat stable, and murine heat stable) and adhesins (K88, K99, and 987P antigens) of 149 piglets from different herds in Sweden were investigated. Three categories were investigated: healthy piglets, diarrheal piglets born to unvaccinated dams, and diarrheal piglets born to dams vaccinated with a polyvalent Formalin-killed whole-cell vaccine containing K88 antigen (Porcovac; Hoechst Pharmaceuticals, Hounslow, England). Piglets less than 1 week old and those 1 to 8 weeks old were evaluated separately. Diarrheal piglets less than 1 week old from vaccinated dams yielded a higher incidence of K99 antigen-positive E. coli of the murine heat-stable enterotoxigenicity type compared with piglets of the same age group from unvaccinated dams. The percentage of diarrheal cases from which E. coli lacking recognized virulence attributes were isolated was also higher in the former compared with the latter group. In the 1- to 8-week-old diarrheal piglets of vaccinated dams, the overall incidence, enterotoxigenicity type, and serotype of the E. coli isolates resembled those of diarrheal piglets less than 1 week of age from unvaccinated herds. Enterotoxigenic E. coli bearing 987P antigen detectable in vitro was rare. Most of the enterotoxigenic isolates lacking K88, K99, and 987P antigens produced only ST. The investigation pinpoints some of the inadequacies of vaccines of the type studied under field conditions.

Effect of parenteral vaccination of dams on intestinal Escherichia coli in piglets with diarrhea.

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Differences in hydrophobic surface characteristics of porcine enteropathogenic Escherichia coli with or without K88 antigen as revealed by hydrophobic interaction chromatography.

Effect of parenteral vaccination of dams on intestinal Escherichia coli in piglets with diarrhea.