Size analysis of polymer beads is currently carried out using instruments such as Coulter counters. Here, image analysis software was used to analyze digital pictures of polymer beads and determine size profiles. Analysis was automated to provide high throughput scanning of images, and results compared favorably with traditional laser diffraction measurements. The technique also enabled the roundness of the beads to be measured in order to assess the bead quality after polymerization and physical manipulation. This method enabled automated and high throughput characterization of beads.

Automated image analysis of polymer beads and size distribution

Publisher Summary This chapter discusses the fundamentals of ion-exchange chromatography. Ion exchangers can be defined as polyvalent materials that are insoluble in water, contain bound ionogenic groups, and are capable of dissociating and exchanging ions in solution. Sometimes the shortened term ionex is used instead of ion exchanger. In spite of the fact that there are natural ion exchangers, most ion exchangers have been prepared synthetically. Ion exchangers and their properties have been described in thousands of papers and tens of monographs that deal with chromatographic aspects. In a typical ion exchanger, there are two main components—a porous matrix (or network) and electrically charged, covalently bound functional ionogenic groups. Ion exchangers can be divided into four main groups, depending on the composition of the matrix: (1) inorganic exchangers, based on aluminum silicates and other suitable minerals, (2) the synthetic resins of many types, (3) ion-exchange cellulose, and (4) ion-exchange polydextran.

Chapter 6 Fundamentals of ion-exchange chromatography

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Chapter 6 Fundamentals of ion-exchange chromatography