11 Papers
26 Citations
Norbert Auer is an academic researcher from University of Natural Resources and Life Sciences, Vienna. The author has contributed to research in topics: Chinese hamster ovary cell & Gene. The author has an hindex of 7, co-authored 11 publications.
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Papers
Comprehensive genome and epigenome characterization of CHO cells in response to evolutionary pressures and over time.
Julia Feichtinger,Inmaculada Hernandez,Christoph Fischer,Michael Hanscho,Norbert Auer,Matthias Hackl,Vaibhav Jadhav,Martina Baumann,Peter M. Krempl,Christian Schmidl,Matthias Farlik,Michael Schuster,Angelika Merkel,Andreas Sommer,Simon Heath,Daniel Rico,Christoph Bock,Gerhard G. Thallinger,Nicole Borth +18 more
TL;DR: Comprehensive genome and epigenome data are presented for six related CHO cell lines, both in response to perturbations (different culture conditions and media as well as selection of a specific phenotype with increased transient productivity) and in steady state (prolonged time in culture under constant conditions).
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Changes in Chromosome Counts and Patterns in CHO Cell Lines upon Generation of Recombinant Cell Lines and Subcloning
Sabine Vcelar,Michael Melcher,Norbert Auer,Astrid Hrdina,Anja Puklowski,Friedrich Leisch,Vaibhav Jadhav,Till Wenger,Martina Baumann,Nicole Borth +9 more
TL;DR: It is concluded that the process of subcloning itself does not contribute to an improved karyotypic homogeneity of a population, while the selection for a specific cell property inherently can provide evolutionary pressure that may lead to improved chromosomal stability as well as to a more homogenous population.
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Epigenetic regulation of gene expression in Chinese Hamster Ovary cells in response to the changing environment of a batch culture
Inmaculada Hernandez,Inmaculada Hernandez,Heena Dhiman,Gerald Klanert,Vaibhav Jadhav,Norbert Auer,Michael Hanscho,Martina Baumann,Anna Esteve-Codina,Marc Dabad,Jessica Gómez,Tyler Alioto,Angelika Merkel,Emanuele Raineri,Simon Heath,Daniel Rico,Nicole Borth +16 more
TL;DR: The coding genes with the highest degree of variation in expression during the batch culture are characterized by a larger number of possible triplex‐forming interactions with differentially expressed lncRNAs, which indicates a specific role of lncRNA‐triplexes in enabling rapid and large changes in transcription.
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Transcriptomic changes in CHO cells after adaptation to suspension growth in protein-free medium analysed by a species-specific microarray.
Smriti Shridhar,Gerald Klanert,Norbert Auer,Inmaculada Hernandez-Lopez,Maciej M. Kańduła,Matthias Hackl,Johannes Grillari,Nancy Stralis-Pavese,David P. Kreil,Nicole Borth +9 more
TL;DR: A publicly available microarray design is constructed for effective genome-scale expression profiling in CHO cells based on the recent release of CHO and Chinese Hamster genome sequences, including an annotated RefSeq genome.
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Effect of CyDye minimum labeling in differential gel electrophoresis on the reliability of protein identification.
TL;DR: The migration differences of labeled and unlabeled species are quantified together with the impact of this effect on the certainty of protein identification and sequence coverage investigating proteins up to 90 kDa.
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