Nikolaus Weber
6 Papers
220 Citations
Nikolaus Weber is an academic researcher. The author has contributed to research in topics: Plant cell & Enzyme. The author has an hindex of 5, co-authored 6 publications.
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Papers
A simple enzymatic method for the preparation of radiolabeled erucoyl-CoA and other long-chain fatty acyl-CoAs and their characterization by mass spectrometry☆
TL;DR: Fast ion bombardment-mass spectrometry was employed to confirm the structures of the various acyl-CoAs and yields were greater than 90% and the purities greater than 95%, based on the distribution of radioactivity, and chromatographic and spectral properties.
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Biosynthesis of Acyl Lipids Containing Very-Long Chain Fatty Acids in Microspore-Derived and Zygotic Embryos of Brassica napus L. cv Reston
David C. Taylor,Dennis L. Barton,Kevin P. Rioux,Samuel L. MacKenzie,Darwin W. Reed,E. W. Underhill,M. Keith Pomeroy,Nikolaus Weber +7 more
TL;DR: The biosynthesis of VLCFAs and their incorporation into glycerolipids in vitro in the MD embryo system occurred at rates comparable to those measured in developing zygotic Reston embryos at about 20 days postanthesis and the incorporation of newly synthesized V LCFAs into TAGs via the Kennedy pathway was demonstrated.
Triacylglycerol Bioassembly in Microspore-Derived Embryos of Brassica napus L. cv Reston'
David C. Taylor,Nikolaus Weber,Dennis L. Barton,E. W. Underhill,Lawrence Hogge,Randall J. Weselake,M. Keith Pomeroy +6 more
TL;DR: The rate of 22:1-CoA:DGAT activity in vitro could more than account for the maximal rate of TAG biosynthesis observed in vivo during embryo development.
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Formation of trierucoylglycerol (Trierucin) from 1,2-dierucoylglycerol by a homogenate of microspore-derived embryos of Brassica napus L
TL;DR: Stereospecific analysis of radioactive triacylglycerols revealed that labeled erucoyl moieties had been incorporated exclusively into thesn-1,3 positions with more than 95% of radioactivity in theSn-3 position, which is of particular interest for the genetic modification of rape and other Cruciferae for producing trierucin in their seed oils.
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Biosynthesis of storage lipids in plant cell and embryo cultures.
TL;DR: Both somatic and gametophytic embryos of oil plants exhibit high capacities for storage lipid biosynthesis and accumulation in vitro compared to cultured plant cells, and the microspore-derived embryo system is recommended to both plant breeders and plant biochemists for the selection and multiplication of plants of superior quality.
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