The invention relates to a novel isothermal nucleic acid amplification technology, namely an isothermal multiple self-pairing triggering amplification technology (IMSA) According to the technology, amplification of a pathogeny gene can be realized under the condition of isothermality and under the action of a single functional enzyme without a thermal cycle instrument The result determination of the isothermal multiple self-pairing triggering amplification technology (IMSA) can be realized by simple measures such as observing white precipitate of by-product magnesium pyrophosphate, adding a chromogenic agent and observing the color change before or after amplification The most unique characteristic of the technology is that multiple oligonucleotide structures which can perform self-pairing and then trigger circular amplification will be generated during the process of amplification so that the triggering probability of following circular amplification is markedly increased, the amplification efficiency and detection sensitivity are promoted On the basis of the isothermal multiple self-pairing triggering amplification technology (IMSA) we establish detection method of hand-foot-and-mouth disease pathogen coxsackievirus type A16 (CVA16) and human enterovirus type EV71 (EV71) VP1 gene, and rapid detection method of human influenza a virus H7N9 HA gene By the application of the invention, simple, rapid, highly sensitive and highly specific detection can be realized

Novel isothermal multiple self-pairing triggering amplification technology

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Novel isothermal multiple self-pairing triggering amplification technology