Nicholas Treen
University of Tsukuba
21 Papers
47 Citations
Nicholas Treen is an academic researcher from University of Tsukuba. The author has contributed to research in topics: Ciona & Ciona intestinalis. The author has an hindex of 9, co-authored 18 publications. Previous affiliations of Nicholas Treen include Princeton University & Tohoku University.
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Papers
Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona
Nicholas Treen,Keita Yoshida,Tetsushi Sakuma,Haruka Sasaki,Narudo Kawai,Takashi Yamamoto,Yasunori Sasakura +6 more
TL;DR: It is reported that TALENs are highly effective at inducing mutations in specific genomic loci in the ascidian chordate Ciona intestinalis and knocked out Hox12 in the epidermis and Fgf3 in neural tissues.
Mollusc gonadotropin-releasing hormone directly regulates gonadal functions: a primitive endocrine system controlling reproduction.
Nicholas Treen,Naoki Itoh,Hanae Miura,Ippei Kikuchi,Takenori Ueda,Keisuke G. Takahashi,Takayoshi Ubuka,Kazutoshi Yamamoto,Peter J. Sharp,Kazuyoshi Tsutsui,Makoto Osada +10 more
TL;DR: In this paper, the cDNA transcripts of prepro GnRH-like peptides from two species of bivalve molluscs, the Yesso scallop Patinopecten yessoensis and the Pacific oyster Crassostrea gigas, were cloned and compared with those from other invertebrates and vertebrates, and determined their sites of expression and biological activity.
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Molecular characterization of an estrogen receptor and estrogen-related receptor and their autoregulatory capabilities in two Mytilus species.
Kazue Nagasawa,Nicholas Treen,Reki Kondo,Yurika Otoki,Naoki Itoh,Jeanette M. Rotchell,Makoto Osada +6 more
TL;DR: Evidence that one (ER) of these may have a capacity to autoregulate its own expression in the gonadal cells by estrogen (E2) and that this gene is responsive to estrogenic compounds is presented.
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Molluscan GnRH associated with reproduction.
Makoto Osada,Nicholas Treen +1 more
TL;DR: In cephalopods and gastropods the presence of a GnRH-like peptide was detected with heterologous antibodies and the identified GnRH was suggested to be involved with behavior and reproduction and will provide useful insights into the evolution of reproductive endocrinology.
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Germ cell mutations of the ascidian Ciona intestinalis with TALE nucleases
Keita Yoshida,Nicholas Treen,Akiko Hozumi,Tetsushi Sakuma,Takashi Yamamoto,Yasunori Sasakura +5 more
TL;DR: It is concluded that knockout lines of Ciona that have disrupted target genes can be established through TALEN‐mediated germ cell mutagenesis.
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