The restrictions for halal and vegetarian fermented products apply not only to the food ingredients, but also to the inoculum media. The utilization of a medium for lactic acid bacteria (LAB) leads to some issues from animal-derived proteins sources that may be doubtful for halal and/or vegetarian use. This study aimed to develop a plant-based medium for culturing and maintaining LAB. The result demonstrated that 10 g/L soybean powder in sweet potato extract was suitable for cultivating Lactiplantibacillus plantarum TISTR 2075 with no significant difference (p < 0.05) from MRS (de Man, Rogosa and Sharpe) in the cell number (9.12 log CFU/mL) and specific growth rate (0.04). The feasibility of a plant-based medium to grow and maintain the LAB strains from different origins was evaluated. Compared to MRS, Lpb. plantarum TISTR 2075, Lpb. plantarum MW3, and Lacticaseibacillus casei TISTR 1463 could grow almost as well in a plant-based medium. This medium was also suitable for maintaining the viability of LAB during storage, especially when subjected to slant agar stock culture. It is practical and costs at least 10 times less than MRS. Thus, this study created a low-cost plant-based medium that could be used in laboratories, especially for applications in halal and vegetarian food products.

/pdf/the-potential-of-an-inexpensive-plant-based-medium-for-halal-131q3w6h.pdf

The Potential of an Inexpensive Plant-Based Medium for Halal and Vegetarian Starter Culture Preparation

The effects of different carbon source,nitrogen source,proportion of carbon and nitrogen source, microelement and buffer salts on the growth of Lactobacillus casei Zhang isolated from Koumiss were studied.The enrichment medium for Lactobacillus casei Zhang was optimized by response surface methodology and its composition was glucose 20.9 g/L,soy peptone 10.45 g/L,yeast extract 10.45 g/L,K2HPO4 3.5 g/L, sodium acetate 14.6 g/L,sodium citrate 2.35 g/L,MgSO4·7H2O 1.0 g/L,MnSO4·5H2O 54 mg/L, CuSO4·5H2O 10 mg/L,tween 80 1.0 g/L.After cultivated in enrichment medium for 18h at 37℃,the living cells of Lactobacillus casei Zhang were 4.78×109 CFU/mL,which was about 10 times higher than in MRS (4.8×10 8 CFU/mL).

Study on the Optimization of Enrichment Medium of Lactobacillus casei Zhang

Abstract In this research, the surface activity of bacterial supernatant and cell surface was measured by the method of oil drain ring. The influence of 19 kinds of amino acids (Histidine, Threonine, Valine, Isoleucine, Leucine, Phenylalanine, Arginine, Proline, Methionine, Tryptophan, Alanine, Glycine, Glutamine, Cysteine, Hydroxyproline, Valine, Asparagine, Proline, Glutamine, Serine, and Glutamic acid, Hydroxyproline, Tyrosine) on the production of biosurfactant by Pediococcus acidilactici F70 was studied by single factor experiment, and the main amino acids promoting the production of biosurfactant were selected by Plackett–Burman design. The results showed that the yield of biological surfactant with added amino acid increased, and the yield of Glutamine produced biosurfactant in the supernatant was the highest, which was two times higher than that of the control group (414.00mg/L); the yield of biosurfactant on the cell surface was the highest when Arginine was added, which was three times higher than that of the control group. In the end, 8 of the 19 amino acids (Glycine, Tryptophan, Proline, Methionine, Arginine, Leucine, Serine and Alanine) were selected to promote the production of biosurfactants of Pediococcus acidilactici F70. The results of Plackett–Burman design showed that Alanine, Proline and Leucine had significant effects on the production of biosurfactants.

/pdf/effect-of-amino-acids-on-the-production-of-biosurfactant-by-encujx6gl7.pdf

Effect of Amino Acids on the Production of Biosurfactant by Pediococcus Acidilactici F70

Abstract Cell envelope proteases (CEPs) can break down milk protein into peptides with different functions, which are of great benefit to human health. Therefore, the high-yield CEPs of Lactobacillus plantarum have the potential to produce functional dairy products. In previous experiments, we found that Na2HPO4, inulin, casein peptone and leucine have significant effects on CEP production by Lactobacillus plantarum LP69. So we proceeded to optimize the composition of the CEP-producing culture medium of L. plantarum through Box-Behnken design and response surface methodology. The protease activity, protein content and specific activity of CEPs produced by L. plantarum by inulin (0.2, 0.3, 0.4 %), casein peptone (0.4, 0.6, 0.8 %), Na2HPO4 (0.50, 0.52, 0.54 %) and leucine (14, 16, 18 mg/L) were evaluated. The optimal ratio of medium is 0.4 % inulin, 0.66 % casein peptone, 0.5 % Na2HPO4 and 14.04 mg/L Leucine. The final enzyme activity reached (24.46±0.81) U/mL, and the specific activity reached (1.41±0.46) U/mg.

Effect of medium composition on cell envelope proteinase production by Lactobacillus plantarum LP69

This research evaluated a bacterial strain, Bacillus cereus (S55), isolated from the soil for its bioflocculant production capabilities. The strain was identified through morphological and 16s rRNA gene sequencing. The optimization of culture conditions, using One-factor-at-a-time method, significantly enhanced bioflocculant production with glucose and urea. FTIR analysis showed the presence of hydroxyl, amine and carboxylate functional groups, with polysaccharides as predominant components. Scanning electron microscopy and X-ray diffraction confirmed the crystalline nature of the bioflocculant. The strain studied showed potential in treating household wastewater and was effective at removing dyes, suggesting alternatives for wastewater management.

Screening, characterization, and production of Bacillus cereus (S55) bioflocculant isolated from soil for application in wastewater treatment

In present study, the effects of carbon sources (glucose, lactose, sucrose, galactose, maltose and soluble starch) and nitrogen sources (casein peptone, whey protein, soy peptone, yeast, tryptone, beef extract and peptone) on activity of cell envelope proteinases (CEP), specific activity, protein content, OD₆₀₀ value and pH in MRS broth fermented by Lactobacillus plantarum LP69 were investigated by individual factor experiment. The results indicated that carbon and nitrogen sources have significant influence on the activity of CEP and specific activitys of L. plantarum LP69, glucose, maltose, casein peptone and peptone are superior to other selected carbon and nitrogen sources. The optimum concentrations of glucose, maltose, casein peptone and peptone for L. plantarum LP69 are 2%, 2%, 1% and 1%; the activity of CEP are 19.52U/mL, 21.13U/mL, 13.49U/mL and 20.61U/mL, respectively.

/pdf/effects-of-carbon-and-nitrogen-sources-on-activity-of-cell-wz8lfftxwl.pdf

Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by Lactobacillus plantarum LP69

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Effects of Carbon and Nitrogen Sources on Activity of Cell Envelope Proteinase Produced by Lactobacillus plantarum LP69