Nebat Ali
San Jose State University
6 Papers
36 Citations
Nebat Ali is an academic researcher from San Jose State University. The author has contributed to research in topics: Gene & Cas9. The author has an hindex of 4, co-authored 5 publications.
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Papers
Bright split red fluorescent proteins for the visualization of endogenous proteins and synapses.
Siyu Feng,Aruna Varshney,Doris Coto Villa,Cyrus Modavi,John Kohler,Fatima Farah,Shuqin Zhou,Shuqin Zhou,Nebat Ali,Joachim D. Müller,Miri K. Van Hoven,Bo Huang +11 more
- 17 Sep 2019
TL;DR: A system based on split fluorescent proteins with enhanced complementation efficiency is developed, characterise further the complementation mechanism and illustrate its use by visualising endogenous synapsis in C.elegans though the tagging of endogenous proteins.
C. elegans avoids toxin-producing Streptomyces using a seven transmembrane domain chemosensory receptor.
Alan Tran,Angelina Tang,Colleen T O'Loughlin,Anthony Balistreri,Eric Chang,Doris Coto Villa,Joy Li,Aruna Varshney,Vanessa Jimenez,Jacqueline Pyle,Bryan Tsujimoto,Christopher Wellbrook,Christopher Vargas,Alex Duong,Nebat Ali,Sarah Y Matthews,Samantha Levinson,Sarah Woldemariam,Sami Khuri,Martina Bremer,Daryl K. Eggers,Noelle D. L'Etoile,Laura C Miller Conrad,Miri K. VanHoven +23 more
TL;DR: It is demonstrated that upon exposure to Streptomyces at their head or tail, nematodes display an escape response that is mediated by bacterially produced cues, providing a window into the molecules and organs used in the coevolutionary arms race between predator and potential prey.
Using a Robust and Sensitive GFP-Based cGMP Sensor for Real-Time Imaging in Intact Caenorhabditis elegans.
Sarah Woldemariam,Jatin Nagpal,Tyler Hill,Joy Li,Martin W. Schneider,Raakhee Shankar,Mary Futey,Aruna Varshney,Nebat Ali,Jordan Mitchell,Kristine Andersen,Benjamin Barsi-Rhyne,Alan Tran,Wagner Steuer Costa,Michelle C. Krzyzanowski,Yanxun V. Yu,Chantal Brueggemann,O. Scott Hamilton,Denise M. Ferkey,Miri K. VanHoven,Piali Sengupta,Alexander Gottschalk,Noelle D. L'Etoile +22 more
TL;DR: It is demonstrated that a C. elegans codon-optimized version of the cpEGFP-based cGMP sensor FlincG3 can be used to visualize rapidly changing cG MP levels in living, behaving Caenorhabditis elegans and that the reporter can potentially reveal unexpected spatiotemporal landscapes ofcGMP in response to stimuli.
The receptor protein tyrosine phosphatase CLR-1 is required for synaptic partner recognition.
Aruna Varshney,Kelli Leilani Benedetti,Katherine Watters,Raakhee Shankar,David Tatarakis,Doris Coto Villa,Khristina Magallanes,Venia Agenor,William Wung,Fatima Farah,Nebat Ali,Nghi Le,Jacqueline Pyle,Amber Farooqi,Zanett Kieu,Martina Bremer,Miri K. VanHoven +16 more
TL;DR: This study defines a new mechanism by which SPR is governed, and demonstrates that these three conserved families of molecules, with roles in neurological disorders and cancer, can act together to regulate communication between cells.
Recyclable CRISPR/Cas9 mediated gene disruption and deletions in Histoplasma
TL;DR: In this paper , the authors adapted a CRISPR/Cas9 system to facilitate targeted gene disruption in Histoplasma with high efficiency, and used this system to disrupt a number of target genes including RYP2 and SRE1 where loss-of-function mutations could be monitored visually by colony morphology or color.