Yarrowia lipolytica has been developed as a production host for a large variety of biotechnological applications. Efficacy and safety studies have demonstrated the safe use of Yarrowia-derived products containing significant proportions of Yarrowia biomass (as for DuPont's eicosapentaenoic acid-rich oil) or with the yeast itself as the final product (as for British Petroleum's single-cell protein product). The natural occurrence of the species in food, particularly cheese, other dairy products and meat, is a further argument supporting its safety. The species causes rare opportunistic infections in severely immunocompromised or otherwise seriously ill people with other underlying diseases or conditions. The infections can be treated effectively by the use of regular antifungal drugs, and in some cases even disappeared spontaneously. Based on our assessment, we conclude that Y. lipolytica is a "safe-to-use" organism.

https://www.tandfonline.com/doi/pdf/10.3109/1040841X.2013.770386

Yarrowia lipolytica: safety assessment of an oleaginous yeast with a great industrial potential.

Opportunities for improving biodiesel production via lipase catalysis

Biodiesel has provided an eco-friendly solution to fuel crisis, as it is renewable, biodegradable and a non-toxic fuel that can be easily produced through enzymatic transesterification of vegetable oils and animal fats. Enzymatic production of biodiesel has many advantages over the conventional methods as high yields can be obtained at low reaction temperatures with easy recovery of glycerol. Microbial lipases are powerful biocatalysts for industrial applications including biodiesel production at lower costs due to its potential in hydrolyzing waste industrial materials. Among them, lipases from yeasts, Candida antarctica, Candida rugosa, Cryptococcus sp., Trichosporon asahii and Yarrowia lipolytica are known to catalyze such reactions. Moreover, stepwise addition of methanol in a three step, two step and single step reactions have been developed using yeast lipases to minimize the inhibitory effects of methanol. The latest trend in biodiesel production is the use of whole-cell as biocatalysts, since the process requires no downstream processing of the enzyme. Synthesis of value added products from the byproduct glycerol further reduces the production cost of biodiesel. This review aims at compiling the information on various yeast lipase catalyzed transesterification reactions for greener production of biodiesel.

Enzymatic transesterification for production of biodiesel using yeast lipases: An overview

Current status and new developments of biodiesel production using fungal lipases

Enzymatic hydroesterification has recently attracted research interest because of the high-value products created during biodiesel production. The use of this process overcomes problems related to conventional methods for biodiesel production, such as slow reaction rate and soap formation. The method comprises two basic processes to produce fatty acid alkyl esters from triacylglycerols, namely, enzymatic hydrolysis and enzymatic esterification. Although enzymatic hydroesterification for biodiesel production has many advantages, such as lower energy consumption and converting low-quality feedstock, it has not been used on an industrial scale mainly because of some impediments, including enzyme cost and conversion efficiency. This review presents a comprehensive evaluation of recent investigations on enzymatic hydrolysis and enzymatic esterification to lower process costs and increase yields.

A review of the enzymatic hydroesterification process for biodiesel production

The present invention provides a crude lipase enzyme solution of Yarrowia lipolytica microzyme CGMCC No.2707 and a method for synthesizing biological diesel oil by catalyzing the hydrolization and esterification reaction of waste animal and vegetable fat by the immobilized lipase of the crude lipase enzyme solution. A method for preparing the lipase crude enzyme solution and the immobilized enzyme is also provided. The method uses hydrolization and esterification reactions of the oil and fat use lipase as catalysts, but does not use strong acid and strong base. The method has the advantages of mild reaction condition and small pollution. Organic solvent is not used during the entire production process of the fatty acid methyl ester/ethyl ester. The reaction is safe and the efficiency is high.

Method for synthesizing biodiesel by two-step catalysis of lipid via Yarrowia lipolytica lipase, and biodiesel obtained thereby

Thermochemical energy storage using silica gel: Thermal storage performance and nonisothermal kinetic analysis

Oleic Acid Esterification in Solvent-Free Medium by Yarrowia lipolytica Lipase Immobilized on Fabric Membranes

Flue gas produced by biomass fuel combustion contains various chlorine-containing substances and is an important factor causing biomass boiler corrosion. The corrosion processes of chlorine, hydrogen chloride and water on iron covered with an intact/damaged oxide film were investigated under the high temperature of 1300 K through reactive molecular dynamics simulation. The results show that the diffusion processes of oxygen and chlorine are similar and can be divided into three stages: rapid diffusion, continuous diffusion, and no oxide film (stable). Oxygen diffusion in Fe2O3 into a pure iron layer is the main cause of gas corrosion in iron/iron oxide systems. A complete oxide film can hinder iron corrosion by chlorine and hydrogen chloride. Damage in an oxide film significantly affects oxygen and chlorine diffusion and iron corrosion. However, such influence is gradually reduced. The integrity of a protective film is the key to alleviating corrosion. Water facilitates the dissociation of chlorine and hydrogen chloride, and it reacts with iron at high temperatures to enhance corrosion. This study improves the understanding of the iron oxide/iron corrosion from chlorine-containing gases from a microscopic perspective and is of great significance to metal corrosion protection and biomass combustion technologies.

/pdf/corrosion-of-iron-covered-with-iron-oxide-film-by-chlorine-2l3bpp72.pdf

Corrosion of Iron Covered with Iron Oxide Film by Chlorine and Hydrogen Chloride Gases: A Molecular Dynamics Simulation Study Using the ReaxFF

Enzymatic biodiesel production by transesterification in solvent media has been investigated intensively, but glycerol, as a by-product, could block the immobilized enzyme and excess n-hexane, as a solution aid, would reduce the productivity of the enzyme. Esterification, a solvent-free and no-glycerol-release system for biodiesel production, has been developed, and two-step catalysis of soybean oil, hydrolysis followed by esterification, with Yarrowia lipolytica lipase is reported in this paper. First, soybean oil was hydrolyzed at 40°C by 100 U of lipase broth per 1 g of oil with approximately 30% to 60% (vol/vol) water. The free fatty acid (FFA) distilled from this hydrolysis mixture was used for the esterification of FFA to fatty acid ethyl ester by immobilized lipase. A mixture of 2.82 g of FFA and equimolar ethanol (addition in three steps) were shaken at 30°C with 18 U of lipase per 1 gram of FFA. The degree of esterification reached 85% after 3 hours. The lipase membranes were taken out, dehydrated and subjected to fresh esterification so that over 82% of esterification was maintained, even though the esterification was repeated every 3 hours for 25 batches. The two-step enzymatic process without glycerol released and solvent-free demonstrated higher efficiency and safety than enzymatic transesterification, which seems very promising for lipase-catalyzed, large-scale production of biodiesel, especially from high acid value waste oil.

/pdf/two-step-synthesis-of-fatty-acid-ethyl-ester-from-soybean-2nqp24pfaw.pdf

Two-step synthesis of fatty acid ethyl ester from soybean oil catalyzed by Yarrowia lipolytica lipase.

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