Recently, two alternative targets in insect periphery nerve system have been explored for environmentally-friendly approaches in insect pest management, namely odorant-binding proteins (OBPs) and odorant receptors (ORs). Located in insect antennae, OBPs are thought to be involved in the transport of odorants to ORs for the specific signal transduction of behaviorally active odorants. There is rich information on OBP binding affinity and molecular docking to bioactive compounds as well as ample 3D crystal structures due to feasible production of recombinant proteins. Although these provide excellent opportunities for them to be considered as pest control targets and a tool to design pest control agents, the debates on their binding specificity represent an obstacle. On the other hand, ORs have recently been functionally characterized with increasing evidence for their specificity, sensitivity and functional roles in pest behaviors. However, a major barrier to use ORs for semiochemical discovery is the lack of 3D crystal structures. Thus, OBPs and ORs have not been analyzed comparatively together so far for their feasibility as pest control targets. Here, we summarize the state of OBPs and ORs research in terms of its application in insect pest management. We discuss the suitability of both proteins as pest control targets and their selection toward the discovery of new potent semiochemicals. We argue that both proteins represent promising targets for pest control and can be used to identify new super-ligands likely present in nature and with reduced risk of resistance development than insect pesticides currently used in agriculture. We discuss that with the massive identification of OBPs through RNA-seq and improved binding affinity measurements, these proteins could be reconsidered as suitable targets for semiochemical discovery.

/pdf/odorant-receptors-and-odorant-binding-proteins-as-insect-2tu2xus9oe.pdf

Odorant Receptors and Odorant-Binding Proteins as Insect Pest Control Targets: A Comparative Analysis.

Plant-derived volatile organic compounds (VOCs) play pivotal roles in interactions with insect herbivores. Individual VOCs can be directly toxic or deterrent, serve as signal molecules to attract natural enemies, and/or be perceived by distal plant tissues as a priming signal to prepare for expected herbivory. Environmental conditions, as well as the specific plant-insect interaction being investigated, strongly influence the observed functions of VOC blends. The complexity of plant-insect chemical communication via VOCs is further enriched by the sophisticated molecular perception mechanisms of insects, which can respond to one or more VOCs and thereby influence insect behavior in a manner that has yet to be fully elucidated. Despite numerous gaps in the current understanding of VOC-mediated plant-insect interactions, successful pest management strategies such as push-pull systems, synthetic odorant traps, and crop cultivars with modified VOC profiles have been developed to supplement chemical pesticide applications and enable more sustainable agricultural practices. Future studies in this field would benefit from examining the responses of both plants and insects in the same experiment to gain a more complete view of these interactive systems. Furthermore, a molecular evolutionary study of key genetic elements of the ecological interaction phenotypes could provide new insights into VOC-mediated plant communication with insect herbivores.

Molecular ecology of plant volatiles in interactions with insect herbivores.

Nitenpyram disturbs gut microbiota and influences metabolic homeostasis and immunity in honey bee (Apis mellifera L.).

Odorant binding proteins play a key role in the olfactory system and are involved in the odor perception and discrimination of insects. To investigate the potential physiological functions of SaveOBP9 in Sitobion avenae, fluorescence ligand binding experiments, molecular docking, RNA interference, and behavioral tests were performed. Fluorescence binding assay results showed that SaveOBP9 had broad and high (Ki < 10 μM) binding abilities with most of the wheat volatiles, but was more obvious at pH 7.4 than pH 5.0. The binding sites of SaveOBP9 to the volatiles were predicted well by three-dimensional docking structure modeling and molecular docking. Moreover, S. avenae showed a strong behavioral response with the four compounds of wheat. The reduction in mRNA transcript levels after the RNA interference significantly reduced the expression level of SaveOBP9 and induced the non-significant response of S. avenae to the tetradecane, octanal, decanal, and hexadecane. This study provides evidence that SaveOBP9 might be involved in the chemoreception of wheat volatile organic compounds and can successfully contribute in the integrated management programs of S. avenae.

https://www.mdpi.com/1422-0067/21/21/8331/pdf

An Odorant Binding Protein (SaveOBP9) Involved in Chemoreception of the Wheat Aphid Sitobion avenae.

Sitodiplosis mosellana, a periodic but devastating wheat pest, relies on wheat spike volatiles as a cue in selecting hosts for oviposition. Insect odorant-binding proteins (OBPs) are thought to play essential roles in filtering, binding and transporting hydrophobic odorant molecules to specific receptors. To date, the molecular mechanisms underlying S. mosellana olfaction are poorly understood. Here, three S. mosellana antenna-specific OBP genes, SmosOBP11, 16 and 21, were cloned and bacterially expressed. Binding properties of the recombinant proteins to 28 volatiles emitted from wheat spikes were investigated using fluorescence competitive binding assays. Sequence analysis suggested that these SmosOBPs belong to the Classic OBP subfamily. Ligand-binding analysis showed that all three SmosOBPs preferentially bound alcohol, ester and ketone compounds, and SmosOBP11 and 16 also selectively bound terpenoid compounds. In particular, the three SmosOBPs had high binding affinities (Ki < 20 μmol/L) to 3-hexanol and cis-3-hexenylacetate that elicited strong electroantennogram (EAG) response from female antennae. In addition, SmosOBP11 displayed significantly higher binding (Ki < 8 μmol/L) than SmosOBP16 and 21 to 1-octen-3-ol, D-panthenol, α-pinene and heptyl acetate which elicited significant EAG response, suggesting that SmosOBP11 plays a major role in recognition and transportation of these volatiles. These findings have provided important insight into the molecular mechanism by which S. mosellana specifically recognizes plant volatiles for host selection, and have facilitated identification of effective volatile attractants that are potentially useful for pest monitoring and trapping.

Molecular and functional characterization of three odorant‐binding proteins from the wheat blossom midge, Sitodiplosis mosellana

Odorant-binding proteins (OBPs) are believed to play an important role in olfactory recognition. In this study, expression pattern and fluorescence binding characteristics of MaltOBP13 from the Japanese pine sawyer beetle, Monochamus alternatus Hope, were investigated via qPCR analysis of MaltOBP13 mRNA level and binding assay of MaltOBP13 and ligands. qPCR monitoring indicated MaltOBP13 mainly expressed in newly emerged males, particularly highly expressed in the last abdominal segment of males, and the expression level was significantly higher in 13-day-old mated adults than those of other stages. To further understand the function of the MaltOBP13 protein in odorant reception, the binding affinity of recombinant MaltOBP13 to ligands was tested by fluorescence binding assays with N-phenyl-1-naphthylamine as a fluorescent probe. The results of this assay indicated that MaltOBP13 exhibited a high binding affinity for pine volatiles and binding capacity was higher in acidic conditions than in neutral environment, indicating a possible role in finding host plants.

Expression pattern and ligand‐binding properties of odorant‐binding protein 13 from Monochamus alternatus hope

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Expression pattern and ligand‐binding properties of odorant‐binding protein 13 from Monochamus alternatus hope