The invention discloses an miRNAs multiplex amplification system for identifying tissue origin of human body fluid and a special primer combination thereof. The primer combination comprises a first primer combination and a second primer combination, wherein the first primer combination consists of 16 primers, and the nucleotide sequences are sequentially shown as sequence 21 to sequence 28 and sequence 31 to sequence 38; the second primer combination consists of 8 primers, and the nucleotide sequences are sequentially shown as a sequence 11, a sequence 12, a sequence 13, a sequence 14, a sequence 16, a sequence 17, a sequence 18 or a sequence 19. When the miRNAs multiplex amplification system provided by the invention is used, the tissue origin of human body fluid can be effectively identified; the steps for identifying the tissue origin of forensic field body fluid or spots are greatly simplified; an accurate scientific basis can be provided for determining the case character, determining suspects, realizing the conviction and sentencing and the like. Important application values are realized.

MiRNAs multiplex amplification system for identifying tissue origin of human body fluid and special primer combination thereof

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MiRNAs multiplex amplification system for identifying tissue origin of human body fluid and special primer combination thereof