Mingyue He
Babraham Institute
63 Papers
915 Citations
Mingyue He is an academic researcher from Babraham Institute. The author has contributed to research in topics: Ribosome display & Chemistry. The author has an hindex of 20, co-authored 51 publications.
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Papers
Antibody-Ribosome-mRNA (ARM) Complexes as Efficient Selection Particles for in vitro Display and Evolution of Antibody Combining Sites
Mingyue He,Michael J. Taussig +1 more
TL;DR: A rapid, eukaryotic, in vitro method for selection and evolution of antibody combining sites using antibody-ribosome-mRNA (ARM) complexes as selection particles, which has the potential to be applied equally for selection of receptors or peptides from libraries.
326
Single Step Generation of Protein Arrays From DNA by Cell-Free Expression and in Situ Immobilisation (PISA Method)
Mingyue He,Michael J. Taussig +1 more
TL;DR: It is shown that human single-chain antibody fragments and an enzyme (luciferase) can be functionally arrayed by the PISA method, and this method can overcome problems of insolubility or degradation associated with bacterial expression of recombinant proteins.
244
Printing protein arrays from DNA arrays.
Mingyue He,Oda Stoevesandt,Oda Stoevesandt,Elizabeth A. Palmer,Elizabeth A. Palmer,Farid Khan,Farid Khan,Olle Ericsson,Michael J. Taussig,Michael J. Taussig +9 more
TL;DR: DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.
Double-Hexahistidine Tag with High-Affinity Binding for Protein Immobilization, Purification, and Detection on Ni−Nitrilotriacetic Acid Surfaces
TL;DR: A double-hexahistidine (His6) tag sequence, comprising two hexahistidines separated by an 11-amino acid spacer, which shows at least 1 order of magnitude stronger binding to Ni-NTA-modified surfaces than a conventional single-His6 tag or two single- his6 tags at N- and C-termini is described.
185
In situ synthesis of protein arrays.
TL;DR: In situ or on-chip protein array methods use cell free expression systems to produce proteins directly onto an immobilising surface from co-distributed or pre-arrayed DNA or RNA, enabling protein arrays to be created on demand.
110