Min Shen
Amgen
6 Papers
6 Citations
Min Shen is an academic researcher from Amgen. The author has contributed to research in topics: Endoplasmic reticulum & Secretory pathway. The author has an hindex of 5, co-authored 6 publications. Previous affiliations of Min Shen include Protein Sciences.
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Papers
Interleukin-36 (IL-36) ligands require processing for full agonist (IL-36α, IL-36β, and IL-36γ) or antagonist (IL-36Ra) activity.
Jennifer E. Towne,Blair R. Renshaw,Jason Douangpanya,Brian P. Lipsky,Min Shen,Christopher A. Gabel,John E. Sims +6 more
TL;DR: It is demonstrated that IL-36Ra antagonist activity is dependent upon removal of its N-terminal methionine, and the mechanism of action is directly analogous to that of IL-1Ra, which suggests that protease(s) that activate IL- 36 cytokines could be excellent drug targets for psoriasis.
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Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31
Min Shen,Sophia Siu,Samantha Byrd,Kurt H. Edelmann,Neha Patel,Randal R. Ketchem,Christopher Mehlin,Heather A. Arnett,Haruki Hasegawa +8 more
TL;DR: Various functions that cysteines perform during IL-31 biosynthesis and secretion are illustrated, but their potential roles in cytokine effector functions are highlighted.
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In Vivo Crystallization of Human IgG in the Endoplasmic Reticulum of Engineered Chinese Hamster Ovary (CHO) Cells
Haruki Hasegawa,John Wendling,Feng He,Egor Trilisky,Riki Stevenson,Heather Franey,Francis Kinderman,Gary Li,Deirdre Murphy Piedmonte,Osslund Timothy D,Min Shen,Randal R. Ketchem +11 more
TL;DR: It is shown that the ER-to-Golgi transport steps became rate-limiting in cells with high secretory activity, and the efficiency of IgG protein synthesis and oxidative folding exceeded the capacity of ER export machinery.
Enhancing Antibody Fc Heterodimer Formation through Electrostatic Steering Effects: APPLICATIONS TO BISPECIFIC MOLECULES AND MONOVALENT IgG
Kannan Gunasekaran,Martin Pentony,Min Shen,Logan Garrett,Carla Forte,Anne Woodward,Soo Bin Ng,Teresa L. Born,Marc W. Retter,Kathy Manchulenko,Heather Sweet,Ian Foltz,Michael Wittekind,Wei Yan +13 more
TL;DR: This work modified the CH3 domain interface of the antibody Fc region with selected mutations so that the engineered Fc proteins preferentially form heterodimers, demonstrating the feasibility of robust production of novel Fc-based heterodimeric molecules.
Modulation of in vivo IgG crystallization in the secretory pathway by heavy chain isotype class switching and N-linked glycosylation
Haruki Hasegawa,Carla Forte,Irene Barber,Shanon Turnbaugh,Janelle Stoops,Min Shen,Ai Ching Lim +6 more
TL;DR: An IgG's in vivo crystal morphology and crystallization propensity can be modulated by the properties genetically and biochemically encoded in the HC constant region, and alterations to the constant domain-encoded properties revealed their modulatory roles in CB-inducing propensities and CB morphology.