Michiel Op De Beeck
Lund University
25 Papers
6 Citations
Michiel Op De Beeck is an academic researcher from Lund University. The author has contributed to research in topics: Biology & Paxillus involutus. The author has an hindex of 11, co-authored 16 publications. Previous affiliations of Michiel Op De Beeck include University of Hasselt.
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Papers
Comparison and validation of some ITS primer pairs useful for fungal metabarcoding studies.
Michiel Op De Beeck,Bart Lievens,Pieter Busschaert,Stéphan Declerck,Jaco Vangronsveld,Jan V. Colpaert +5 more
TL;DR: Results indicate that experimental evaluation of primers provides valuable information that could aid in the selection of suitable primers for fungal metabarcoding studies and show that the ITS86F/ITS4 primer pair outperforms other primer pairs tested in terms of in silico primer efficiency, PCR efficiency, coverage, number of reads and number of species-level operational taxonomic units (OTUs) obtained.
Structural variability and niche differentiation in the rhizosphere and endosphere bacterial microbiome of field-grown poplar trees
Bram Beckers,Michiel Op De Beeck,Michiel Op De Beeck,Nele Weyens,Wout Boerjan,Jaco Vangronsveld +5 more
TL;DR: Understanding the complex host–microbe interactions of Populus could provide the basis for the exploitation of the eukaryote–prokaryote associations in phytoremediation applications, sustainable crop production (bio-energy efficiency), and/or the production of secondary metabolites.
Comparative Evaluation of Four Bacteria-Specific Primer Pairs for 16S rRNA Gene Surveys.
Sofie Thijs,Michiel Op De Beeck,Bram Beckers,Sascha Truyens,Vincent Stevens,Jonathan D. Van Hamme,Nele Weyens,Jaco Vangronsveld +7 more
TL;DR: Insilico evaluation of four bacterial primers for characterizing complex microbial communities in explosives contaminated and non-contaminated forest soil and by in silico evaluation against the current SILVA123 database suggests the high utility of primer pair 341f/785r for soil and plant-associated bacterial microbiome studies.
Performance of 16s rDNA Primer Pairs in the Study of Rhizosphere and Endosphere Bacterial Microbiomes in Metabarcoding Studies
Bram Beckers,Michiel Op De Beeck,Sofie Thijs,Sascha Truyens,Nele Weyens,Wout Boerjan,Wout Boerjan,Jaco Vangronsveld +7 more
TL;DR: It is shown that primer pair 799F-1391R outperforms all other primer pairs in the study of the bacterial microbiomes present in the rhizosphere soil, root, stem and leaf endosphere of field-grown poplar trees and in the elimination of non-target DNA and retrieval of bacterial OTUs.
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Lignin engineering in field-grown poplar trees affects the endosphere bacterial microbiome
Bram Beckers,Michiel Op De Beeck,Michiel Op De Beeck,Nele Weyens,Rebecca Van Acker,Marc Van Montagu,Wout Boerjan,Jaco Vangronsveld +7 more
TL;DR: The host genotype modulation of the plant microbiome by minute genetic variations in the plant genome is demonstrated, demonstrating the full consequences of plant metabolic pathway engineering and its relation with the environment and the intended genetic improvement.
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