Michal Hammel
Lawrence Berkeley National Laboratory
117 Papers
190 Citations
Michal Hammel is an academic researcher from Lawrence Berkeley National Laboratory. The author has contributed to research in topics: Small-angle X-ray scattering & Chemistry. The author has an hindex of 42, co-authored 101 publications. Previous affiliations of Michal Hammel include Comenius University in Bratislava & University of California, San Francisco.
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Papers
X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
TL;DR: In this article, a review of the use of small angle X-ray scattering (SAXS) for modeling macromolecular folding, unfolding, aggregation, extended conformations, flexibly linked domains, shape, conformation, and assembly state in solution, albeit at the lower resolution range of about 50 A to 10 A resolution, is presented.
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Structural Insights into NEDD8 Activation of Cullin-RING Ligases: Conformational Control of Conjugation
TL;DR: Results point to a conformational control of CRL activity, with ligation of NEDD8 shifting equilibria to disfavor inactive CAND1-bound closed architectures, and favor dynamic, open forms that promote polyubiquitination.
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Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS)
Greg L. Hura,Angeli Lal Menon,Michal Hammel,Robert P. Rambo,Farris L. Poole,Susan E. Tsutakawa,Francis E. Jenney,Francis E. Jenney,Scott Classen,Kenneth A. Frankel,Robert C. Hopkins,Sung-Jae Yang,Joseph W. Scott,Bret D. Dillard,Michael W. W. Adams,John A. Tainer +15 more
TL;DR: The SAXS pipeline combines automated sample handling of microliter volumes, temperature and anaerobic control, rapid data collection and data analysis, and couples structural analysis with automated archiving to create an efficient pipeline enabling high-throughput analysis of protein structure in solution with small angle X-ray scattering.
Accurate SAXS profile computation and its assessment by contrast variation experiments.
TL;DR: FoXS utility for analyzing heterogeneous samples was demonstrated for intrinsically flexible XLF-XRCC4 filaments and Ligase III-DNA complex and a significant correlation of the SAXS score with the accuracy of a structural model was demonstrated.
574
FoXS: a web server for rapid computation and fitting of SAXS profiles
TL;DR: FoXS (Fast X-Ray Scattering) is a rapid method for computing a SAXS profile of a given structure and for matching of the computed and experimental profiles.