Michael Wallisch
University of Würzburg
3 Papers
14 Citations
Michael Wallisch is an academic researcher from University of Würzburg. The author has contributed to research in topics: Termination factor & Terminator (genetics). The author has an hindex of 3, co-authored 3 publications.
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Papers
Ku antigen supports termination of mammalian rDNA replication by transcription termination factor TTF-I.
TL;DR: Recombinant Ku protein, purified as heterodimer from coinfected insect cells, is able to partially rescue the barrier activity in Kudepleted HeLa cell extracts, demonstrating that transcription termination factor I and Ku act synergistically to prevent headon collision between the replication and the transcription machinery.
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Termination of Mammalian rDNA Replication: Polar Arrest of Replication Fork Movement by Transcription Termination Factor TTF-I
Josef-Karl Gerber,Eric Gögel,Christian Berger,Michael Wallisch,Friedemann Müller,Ingrid Grummt,Friedrich Grummt +6 more
TL;DR: The same factor that blocks elongating RNA polymerase I prevents head-on collision between the DNA replication apparatus and the transcription machinery and is reproduced in vitro in HeLa cell extracts using 3' terminal murine rDNA fused to an SV40 origin-based vector.
RNA polymerase I transcription termination: Similar mechanisms are employed by yeast and mammals
TL;DR: It is shown that, similar to TTF-I, Reb1p was sufficient for pausing of Pol I from either species, but was unable to cause release of the nascent transcripts from the paused ternary complex.