Michael Piontek
12 Papers
201 Citations
Michael Piontek is an academic researcher. The author has contributed to research in topics: Arxula adeninivorans & Yeast. The author has an hindex of 8, co-authored 12 publications.
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Papers
High-level secretion of hirudin by Hansenula polymorpha —authentic processing of three different preprohirudins
U. Weydemann,P. Keup,Michael Piontek,A. W. M. Strasser,J. Schweden,Gerd Gellissen,Zbigniew A. Janowicz +6 more
TL;DR: A DNA sequence coding for a subtype of the hirudin variant HV1 was expressed in the methylotrophic yeast Hansenula polymorpha from a strongly inducible promoter element derived from a genes of the methanol metabolism pathway and the majority of the secreted product was identified as the full-length 65-amino-acid hirUDin.
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Two novel gene expression systems based on the yeasts Schwanniomyces occidentalis and Pichia stipitis.
TL;DR: Two non-Saccharomyces yeasts have been developed as hosts for heterologous gene expression control in both species and homologous elements derived from highly expressed genes of the respective hosts appeared to be of superior quality.
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Recombinant Hansenula polymorpha as a biocatalyst: coexpression of the spinach glycolate oxidase (GO) and the S. cerevisiae catalase T (CTT1) gene.
Gerd Gellissen,Michael Piontek,U. Dahlems,V. Jenzelewski,John E. Gavagan,Robert DiCosimo,David L. Anton,Zbigniew A. Janowicz +7 more
TL;DR: Rec recombinant strains have been engineered with multiple copies of expression cassettes containing the glycolate oxidase gene from spinach and the catalase T (CTT1) gene from S. cerevisiae to provide options to design this yeast as an industrial biocatalyst in procedures where several enzymes are required for the efficient conversion of a given inexpensive compound into a valuable product.
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Xplor® 2—an optimized transformation/expression system for recombinant protein production in the yeast Arxula adeninivorans
TL;DR: A new Arxula vector system, Xplor 2, for heterologous gene expression was established, which allows the construction of expression plasmids for supertransformation of A. adeninivorans strains secreting target proteins of biotechnological interest and the integration of small vector cassettes consisting of yeast DNA sequences only.
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Large-scale production of tannase using the yeast Arxula adeninivorans.
Erik Böer,Friederike Sophie Breuer,Michael Weniger,Sylvia Denter,Michael Piontek,Gotthard Kunze +5 more
TL;DR: In the present study, transgenic Arxula adeninivorans strains were optimised for tannase production and the effect of fed-batch fermentation on tann enzyme productivity was investigated in detail.
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