Michael Agne
University of Freiburg
6 Papers
3 Citations
Michael Agne is an academic researcher from University of Freiburg. The author has contributed to research in topics: Chemistry & Catalysis. The author has an hindex of 3, co-authored 4 publications.
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Papers
Modularized CRISPR/dCas9 effector toolkit for target-specific gene regulation.
Michael Agne,Ilona Blank,Alica J. Emhardt,Christoph G Gäbelein,Fenja Gawlas,Nadine Gillich,Patrick Gonschorek,Thomas J Juretschke,Stefan D Krämer,Natalie Louis,Anne Müller,Alina Rudorf,Lisa M Schäfer,Manuel C. Scheidmann,Lisa J Schmunk,Philipp Schwenk,Maximilian R Stammnitz,Philipp M Warmer,Wilfried Weber,Adrian Fischer,Beate Kaufmann,Hanna J. Wagner,Gerald Radziwill +22 more
TL;DR: A standardized toolkit utilizing an engineered CRISPR/Cas9 system that enables customizable gene regulation in mammalian cells is developed, which comprises a modular RNAimer plasmid, which encodes the required noncoding RNA components.
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Functional Characterization of Three Specific Acyl-Coenzyme A Synthetases Involved in Anaerobic Cholesterol Degradation in Sterolibacterium denitrificans Chol1S.
Markus Warnke,Tobias Jung,Christian Jacoby,Michael Agne,Franziska Maria Feller,Bodo Philipp,Wolfgang Seiche,Bernhard Breit,Matthias Boll +8 more
TL;DR: Experimental evidence is provided that anaerobic steroid degradation proceeds via numerous alternate CoA-ester-dependent or -independent enzymatic reaction sequences as a result of aldolytic side chain and hydrolytic sterane ring C—C bond cleavages.
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The missing enzymatic link in syntrophic methane formation from fatty acids.
Michael Agne,Sebastian Estelmann,Carola S. Seelmann,Johannes W. Kung,Dennis Wilkens,Hans-Georg Koch,Chris van der Does,Sonja-Verena Albers,Christoph von Ballmoos,Jörg Simon,Matthias Boll +10 more
TL;DR: In this paper, a previously noncharacterized membrane-bound oxidoreductase (EMO) from containing two heme cofactors and 8-methylmenaquinone was identified as key redox components of the redox loop-driven reduction of CO by acyl-coenzyme A (CoA).
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Enoyl-Coenzyme A Respiration via Formate Cycling in Syntrophic Bacteria
TL;DR: It is shown that the oxidative and reductive branches of this pathway are connected via formate cycling involving an energy-conserving redox-loop and a novel type of energy metabolism, referred to as enoyl-CoA respiration, generates a proton motive force via a methylmenaquinone-dependent redoxloop.
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