New insights into the vertical distribution and microbial degradation of microplastics in urban river sediments.

The current status on the taxonomy of Pseudomonas revisited: An update.

Concordance between whole-cell matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry and multilocus sequence analysis approaches in species discrimination within the genus Pseudomonas.

Pseudomonas lutea OK2T (=LMG 21974T, CECT 5822T) is the type strain of the species and was isolated from the rhizosphere of grass growing in Spain in 2003 based on its phosphate-solubilizing capacity. In order to identify the functional significance of phosphate solubilization in Pseudomonas Plant growth promoting rhizobacteria, we describe here the phenotypic characteristics of strain OK2T along with its high-quality draft genome sequence, its annotation, and analysis. The genome is comprised of 5,647,497 bp with 60.15 % G + C content. The sequence includes 4,846 protein-coding genes and 95 RNA genes.

/pdf/high-quality-draft-genome-sequence-of-the-type-strain-of-5d44iqz0pc.pdf

High quality draft genome sequence of the type strain of Pseudomonas lutea OK2(T), a phosphate-solubilizing rhizospheric bacterium.

In this study, we identified a novel Gram-stain-negative, aerobic, motile, and rod-shaped bacterium, strain KX 20T, isolated from the deep seawater in Okinawa Trough, northwestern Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain KX 20T was related to members of the genus 
Pseudomonas
and shares the highest sequence identities with 
Pseudomonas aestusnigri
CECT 8317T (99.4 %) and 
Pseudomonas pachastrellae
JCM 12285T (98.5 %). The 16S rRNA gene sequence identities between strain KX 20T and other members of the genus Pseudomonaswere below 96.6 %. The gyrB and rpoD genes of strain KX 20T shared 82.0 to 89.3 % sequence identity with the gyrB and rpoD genes of the closest phylogenetic neighbours of KX 20T. The predominant cellular fatty acids of strain KX 20T were summed feature 8 (C18 : 1
ω7c and/or C18 : 1
ω6c) (29.2 %), C16 : 0 (24.5 %), summed feature 3 (C16 : 1
ω7c and/or C16 : 1
ω6c) (21.5 %) and C12 : 0 (8.2 %). The major polar lipids of strain KX 20T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and unknown phospholipids. The genomic DNA G+C content of strain KX 20T was 62.9 mol%. On the basis of phylogenetic analysis and phenotypic characteristics, a novel species, Pseudomonas oceani sp. nov. is proposed. The type strain is KX 20T (=CGMCC 1.15195T=DSM 100277T).

Pseudomonas oceani sp. nov., isolated from deep seawater.

Two strains of gram-negative bacteria isolated because of their abilities to decompose xenobiotic compounds were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence analysis, the two strains were found to belong to the genus Pseudomonas. Benzene degrading strain DSM 8628T was moderately related to P. flavescens NCPP 3063T (98.3% similarity), P. monteilii CIP 104883T, and P. plecoglossicida FPC 951T (98.1%). Strain DSM 9751T capable to grow with cetyltrimethylammonium chloride as the sole carbon source showed the highest similarity values with P. tremae CFBP 2341T and P. meliae MAFF 301463T (98.0%), both related to Pseudomonas syringae. The fatty acid pattern of strain DSM 8628T was distinct from patterns of other members of the genus Pseudomonas in combining a high ratio of 3OH-C12:1 (5.1%), a low ratio of 2OH-C12:0 (0.2%) and a relatively low ratio of C18:1ω7c (23.8%). On the basis of phylogenetic analysis, physiological properties and the composition of whole cell fatty acids, two novel species, Pseudomonas benzenivorans sp. nov. with the type strain DSM 8628T (=CIP 109857T) and Pseudomonas saponiphila sp. nov. with the type strain DSM 9751T (=CIP 109856T), are proposed.

Pseudomonas benzenivorans sp. nov. and Pseudomonas saponiphila sp. nov., Represented by Xenobiotics Degrading Type Strains

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Pseudomonas benzenivorans sp. nov. and Pseudomonas saponiphila sp. nov., Represented by Xenobiotics Degrading Type Strains