Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs Use of a portable instrument may reduce the hazard to an operator by reducing an operator's contact with a sample for analysis The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system

Integration of fluids and reagents into self-contained cartridges containing sensor elements

A microarrayer for spotting solution onto a receiving surface in an automated microarray dispensing device. Elements of the invention include: at least one dispense head(6,40) for spotting the receiving surface (4A1-4E10), at least one light source (13) capable of illuminating the receiving surface, at least one camera (12) operating in conjunction with the at least one light source. The camera is capable of acquiring and transmitting surface image data to a computer. The computer is programmed to receive the surface image data and analyze it. The computer then generates post analysis data based on the analysis of the surface image data. The post analysis data is available for improving the spotting of the solution onto the receiving surface. In a preferred embodiment, the surface image data includes information relating to receiving surface alignment, information relating to spot quality, and receiving surface identification information. In a preferred, analysis of the information relating to receiving surface alignment enables the computer to make automatic adjustments to the relative positions of the at least one dispense head and the receiving surface to increase the accuracy of the spotting. In an embodiment, analysis of the information relating to spot quality identifies a spot as pass or fail.

Microarray dispensing with real-time verification and inspection

Methods, systems, and apparatus for detecting the presence of analytes are described. A fluid or gas sample may pass through a microsieve-based detection system and/or a particle- based detection system of a cartridge. Detection and analysis techniques may be applied to determine the identity and quantity of the captured analytes.

Systems and methods including self-contained cartridges with detection systems and fluid delivery systems

A system for the rapid characterization of multi-analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member into which a plurality of cavities may be formed. A series of chemically sensitive particles are, in one embodiment positioned within the cavities. The particles may be configured to produce a signal when a receptor coupled to the particle interacts with the analyte. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized.

Fluid based analysis of multiple analytes by a sensor array

Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator's contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges. Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system.

Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems

A method for counting objects in an image includes the steps of: (a) providing an image including at least one object defined in the image in shades of at least one color; (b) identifying objects in the image having a shade of color which exceeds a threshold shade so as to provide a number of identified objects; (c) evaluating objects of the number of identified objects based upon object criteria to determine whether each object satisfies the criteria and can be counted, or does not satisfy the criteria and is suspect and needs further analysis; (d) repeating steps (b) and (c) for objects which are suspect at shades of color increasingly exceeding the threshold shade until all objects satisfy the criteria; and (e) providing a count of the objects, whereby accuracy of the count is enhanced. Each object is preferably tested for one or more criteria including object size, visibility, color, surface quality and shape.

System for counting colonies of micro-organisms in petri dishes and other culture media

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System for counting colonies of micro-organisms in petri dishes and other culture media