Markus Warnke
University of Freiburg
5 Papers
Markus Warnke is an academic researcher from University of Freiburg. The author has contributed to research in topics: Hydroxylation & Chemistry. The author has an hindex of 5, co-authored 5 publications.
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Papers
Four Molybdenum-Dependent Steroid C-25 Hydroxylases: Heterologous Overproduction, Role in Steroid Degradation, and Application for 25-Hydroxyvitamin D3 Synthesis.
Christian Jacoby,Jens Eipper,Markus Warnke,Oliver Tiedt,Mario Mergelsberg,Hans-Joachim Stärk,Birgit Daus,Zaira Martín-Moldes,María Teresa Zamarro,Eduardo Díaz,Matthias Boll +10 more
TL;DR: A heterologous gene expression platform that allowed for easy isolation and characterization of four highly active S25DH isoenzymes of the DMSO reductase family is established, demonstrating the potential application of the established expression platform for 25-OH-VD3 synthesis and paving the way for the characterization of previously genetically inaccessible S25 DH-like Mo enzymes.
Sporangium Exposure and Spore Release in the Peruvian Maidenhair Fern (Adiantum peruvianum, Pteridaceae).
TL;DR: This work investigated the different processes involved in spore liberation in the polypod fern Adiantum peruvianum (Pteridaceae), and found that the whole sporangium, or parts of it, together with contained spores break away from the false indusium and are shed as a whole.
Functional Characterization of Three Specific Acyl-Coenzyme A Synthetases Involved in Anaerobic Cholesterol Degradation in Sterolibacterium denitrificans Chol1S.
Markus Warnke,Tobias Jung,Christian Jacoby,Michael Agne,Franziska Maria Feller,Bodo Philipp,Wolfgang Seiche,Bernhard Breit,Matthias Boll +8 more
TL;DR: Experimental evidence is provided that anaerobic steroid degradation proceeds via numerous alternate CoA-ester-dependent or -independent enzymatic reaction sequences as a result of aldolytic side chain and hydrolytic sterane ring C—C bond cleavages.
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A patchwork pathway for oxygenase‐independent degradation of side chain containing steroids
Markus Warnke,Christian Jacoby,Tobias Jung,Michael Agne,Mario Mergelsberg,Robert Starke,Nico Jehmlich,Martin von Bergen,Hans-Hermann Richnow,Thomas Brüls,Matthias Boll +10 more
TL;DR: The results indicate that individual molybdopterin-containing steroid dehydrogenases are involved in C25-hydroxylations of steroids with different isoprenoid side chains, followed by the unusual conversion to C26-oic acids.
25‐Hydroxyvitamin D3 Synthesis by Enzymatic Steroid Side‐Chain Hydroxylation with Water
Markus Warnke,Tobias Jung,Juri Dermer,Karin Hipp,Nico Jehmlich,Martin von Bergen,Sascha Ferlaino,Alexander Fries,Michael Müller,Matthias Boll +9 more
TL;DR: This facile and robust method developed for 25OHVD3 synthesis is a novel example for the concept of substrate-engineered catalysis and offers an attractive alternative to chemical or O2 /electron-donor-dependent enzymatic procedures.