Mark L. Collins
Chiron Corporation
26 Papers
1K Citations
Mark L. Collins is an academic researcher from Chiron Corporation. The author has contributed to research in topics: BDNA test & Hepatitis C virus. The author has an hindex of 18, co-authored 26 publications. Previous affiliations of Mark L. Collins include University of California, San Francisco & Bayer Corporation.
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Papers
Patent
Reduction of nonspecific hybridization by using novel base-pairing schemes
Mark L. Collins,Horn Thomas,Sheridan Patrick E,Warner Brian D,Michael S. Urdea +4 more
- 30 Aug 1995
TL;DR: In this paper, a method for reducing background signals encountered in nucleic acid hybridization assays is presented, based on the elimination or significant reduction of the phenomenon of nonspecific hybridization, so as to provide a detectable signal which is produced only in the presence of the target polynucleotide of interest.
251
Accurate quantification of hepatitis C virus (HCV) RNA from all HCV genotypes by using branched-DNA technology.
Jill Detmer,Robert Lagier,J Flynn,C Zayati,Janice A. Kolberg,Mark L. Collins,Mickey S. Urdea,Ray Sanchez-Pescador +7 more
TL;DR: The improved branched DNA assay (HCV RNA 2.0) yielded highly reproducible quantification of hepatitis C virus RNA and displayed a nearly 600-fold dynamic range in quantification up to 120 Meq of HCV RNA per ml.
237
Patent
Solution phase nucleic acid sandwich assays having reduced background noise
Mark L. Collins,Timothy Fultz,Michael S. Urdea,Warner Brian D +3 more
- 07 Dec 1994
TL;DR: In this article, new techniques are provided for substantially reducing background signals encountered in solution phase hybridization assays, which are premised on eliminating or significantly reducing the phenomena of nonspecific hybridization, so as to provide a detectable signal which is produced only in the presence of the target polynucleotide of interest.
197
Preparation and characterization of RNA standards for use in quantitative branched DNA hybridization assays.
Mark L. Collins,C Zayati,Jill Detmer,B. Daly,Janice A. Kolberg,Tai-An Cha,Irvine Bruce,J. Tucker,Mickey S. Urdea +8 more
TL;DR: Standard Hepatitis C virus (HCV) RNAs showed that size has no detectable effect on quantitation in the branched DNA hybridization assay, and standard HCV RNA transcripts were prepared from clones of HCV subtypes 1b and 3a to study the effects of target sequence diversity and probe design on quantification by hybridization.
128
Quantitation of hepatitis G and C viruses in the liver: evidence that hepatitis G virus is not hepatotropic.
Mario G. Pessoa,Norah A. Terrault,Norah A. Terrault,Jill Detmer,Janice A. Kolberg,Mark L. Collins,Howayda M. Hassoba,Howayda M. Hassoba,Teresa L. Wright,Teresa L. Wright +9 more
TL;DR: The liver is not the main site of HGV replication, and the high ratio of HCV RNA levels in liver compared to serum is consistent with its known hepatotropism, but this pattern was not observed for HGV.
127