Mark A. Bernard

TL;DR: This article summarizes research from the laboratory on two aspects of the biochemistry of nucleoside diphosphate kinase from Escherichia coli—first, its interactions with several T4bacteriophage-coded enzymes, as part of a multienzyme complex for deoxyribonucleosidetriphosphate biosynthesis and phenotypes of an E. coli mutant strain carrying a targeted deletion of ndk.

TL;DR: The data suggest a model in which dNTP synthetase complexes, probably more than one per growing DNA chain, are drawn to replication forks via their affinity for gp32 and hence are localized so as to produce dN TPs at their sites of utilization, immediately ahead of growing DNA 3′ termini.

TL;DR: Incorporating normal blood phamacokinetics of T7 phage into in vivo phage display improves the ability to recover targeting peptide motifs and allows effective lead optimization by kSAR.

TL;DR: In this article, the authors present compositions, methods and systems for sequencing a template nucleic acid using a polymerase based, nucleic acids binding reaction involving examination of the interaction between a polymersase and template nuclei acid in the presence of one or more unlabeled nucleotides.