Maren Schiwon
Witten/Herdecke University
11 Papers
6 Citations
Maren Schiwon is an academic researcher from Witten/Herdecke University. The author has contributed to research in topics: CRISPR & Cas9. The author has an hindex of 7, co-authored 10 publications.
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Papers
CRISPR/Cas9 delivery with one single adenoviral vector devoid of all viral genes.
Eric Ehrke-Schulz,Maren Schiwon,Theo Leitner,Stephan David,Thorsten Bergmann,Jing Liu,Anja Ehrhardt +6 more
TL;DR: This work adapted clinically relevant high-capacity adenoviral vectors devoid of all viral genes for the delivery of the CRISPR/Cas9 machinery using a single viral vector to enable fast transfer of the Cas9 gene and gRNA expression units into the HCAdV genome.
One-Vector System for Multiplexed CRISPR/Cas9 against Hepatitis B Virus cccDNA Utilizing High-Capacity Adenoviral Vectors
Maren Schiwon,Eric Ehrke-Schulz,Andreas Oswald,Thorsten Bergmann,Thomas Michler,Ulrike Protzer,Anja Ehrhardt +6 more
TL;DR: High-capacity adenoviral vectors designed to deliver a multiplexed complete CRISPR/Cas9 nuclease system or a complete pair of transcription activator-like effector nucleases directed against the hepatitis B virus (HBV) genome are delivered and its antiviral effect is demonstrated.
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An Engineered Virus Library as a Resource for the Spectrum-wide Exploration of Virus and Vector Diversity.
Wenli Zhang,Jun Fu,Jun Fu,Jing Liu,Hailong Wang,Hailong Wang,Maren Schiwon,Sebastian Janz,Lukas Schaffarczyk,Lukas von der Goltz,Eric Ehrke-Schulz,Johannes Dörner,Manish Solanki,Philip Boehme,Thorsten Bergmann,André Lieber,Chris Lauber,Andreas Dahl,Andreas Petzold,Youming Zhang,A. Francis Stewart,Anja Ehrhardt +21 more
TL;DR: High-throughput screening of the reporter-tagged Ad library in a panel of cell lines including osteosarcoma disease-specific cell lines revealed alternative virus types with enhanced transduction and oncolysis efficiencies, highlighting the usefulness of this resource.
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Viral Vector-Based Delivery of CRISPR/Cas9 and Donor DNA for Homology-Directed Repair in an In Vitro Model for Canine Hemophilia B.
TL;DR: The amplification-refractory mutation system based on qPCR analysis revealed that the single vector application in Huh7-cFIXmut cells resulted in up to 5.52% HDR efficiencies, which was superior to the two-vector strategy.
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Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5.
Eric Ehrke-Schulz,Wenli Zhang,Maren Schiwon,Thorsten Bergmann,Manish Solanki,Jing Liu,Philip Boehme,Theo Leitner,Anja Ehrhardt +8 more
TL;DR: A detailed protocol for efficient cloning and production of HC adenoviral vectors based on the plasmid pAdFTC containing the HCAdV genome is described.
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