Marcus Weinguny
University of Natural Resources and Life Sciences, Vienna
10 Papers
13 Citations
Marcus Weinguny is an academic researcher from University of Natural Resources and Life Sciences, Vienna. The author has contributed to research in topics: Chinese hamster ovary cell & Epigenetics. The author has an hindex of 4, co-authored 9 publications.
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Papers
Directed evolution approach to enhance efficiency and speed of outgrowth during single cell subcloning of Chinese Hamster Ovary cells.
Marcus Weinguny,Gerald Klanert,Peter Eisenhut,Andreas Jonsson,Daniel Ivansson,Ann Lövgren,Nicole Borth +6 more
TL;DR: In this article, an easy way to enhance subclonability using sub-cloning by single cell sorting itself as the selection pressure, resulting in improved subcloning performance of three different host cell lines.
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A CRISPR/Cas9 based engineering strategy for overexpression of multiple genes in Chinese hamster ovary cells.
Peter Eisenhut,Gerald Klanert,Marcus Weinguny,Laurenz J. Baier,Vaibhav Jadhav,Daniel Ivansson,Nicole Borth +6 more
TL;DR: Development and proof-of-concept of a novel engineering strategy using multiplexable activation of artificially repressed genes (MAARGE) that will allow faster screening of overexpression of multiple genes in all possible combinations is presented.
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A cross-species whole genome siRNA screen in suspension-cultured Chinese hamster ovary cells identifies novel engineering targets.
Gerald Klanert,Daniel J. Fernandez,Marcus Weinguny,Peter Eisenhut,Eugen Bühler,Michael Melcher,Steve Titus,Andreas B. Diendorfer,Elisabeth Gludovacz,Elisabeth Gludovacz,Vaibhav Jadhav,Su Xiao,Beate Stern,Madhu Lal,Joseph Shiloach,Nicole Borth +15 more
TL;DR: A cross-species approach is performed by applying a mouse whole-genome siRNA library to CHO cells, optimized the protocol for suspension cultured cells, and developed an in silico method to identify functioning siRNAs, which revealed the limitations of using cross- Species libraries.
How to train your cell - Towards controlling phenotypes by harnessing the epigenome of Chinese hamster ovary production cell lines.
Nicolas Marx,Peter Eisenhut,Marcus Weinguny,Gerald Klanert,Nicole Borth +4 more
TL;DR: In this article , the authors provide an overview about the current understanding of epigenetic regulation in Chinese hamster ovary cells and discuss its significance for shaping the cell's phenotype, and also look into current state-of-the-art technology that can be applied to harness and manipulate the epigenetic network so as to nudge CHO cells towards a specific phenotype.
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Subcloning induces changes in the DNA-methylation pattern of outgrowing Chinese hamster ovary cell colonies
TL;DR: This article showed that the emergence of diverse phenotypes during single cell cloning is associated with changes in DNA methylation patterns and transcriptomes that occur during the sub-cloning process, indicating that epigenetic regulation is a hidden, but important player in cell line development with a major role in the establishment of high performing clones with improved characteristics for bioprocessing.
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