Manjit Singh
DuPont Pioneer
10 Papers
13 Citations
Manjit Singh is an academic researcher from DuPont Pioneer. The author has contributed to research in topics: Hybrid seed & Biology. The author has an hindex of 6, co-authored 10 publications.
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Papers
Concurrent modifications in the three homeologs of Ms45 gene with CRISPR-Cas9 lead to rapid generation of male sterile bread wheat (Triticum aestivum L.).
TL;DR: It was demonstrated that a wild-type copy of Ms45 gene from rice was able to restore fertility to these wheat mutant plants and provide insights into the conservation of MS45 function in a polyploid species.
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Molecular identification of the wheat male fertility gene Ms1 and its prospects for hybrid breeding.
Elise J. Tucker,Ute Baumann,Allan Kouidri,Radoslaw Suchecki,Mathieu Baes,Melissa Garcia,Takashi Okada,Chongmei Dong,Yongzhong Wu,Ajay Sandhu,Manjit Singh,Peter Langridge,Petra Wolters,Marc C. Albertsen,A. Mark Cigan,Ryan Whitford +15 more
TL;DR: It is shown that Ms1 completely restores fertility to ms1d, and encodes a glycosylphosphatidylinositol-anchored lipid transfer protein, necessary for pollen exine development, a key step towards developing a robust hybridization platform in wheat.
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Targeted mutagenesis of a conserved anther-expressed P450 gene confers male sterility in monocots.
A. Mark Cigan,Manjit Singh,Geoffrey Benn,Lanie Feigenbutz,Manish Kumar,Myeong-Je Cho,Sergei Svitashev,Joshua Young +7 more
TL;DR: A custom designed homing endonuclease, Ems26+, was used to generate in planta mutations in the rice, sorghum and wheat orthologs of maize Ms26, which prevents pollen formation resulting in male sterile plants and fertility was restored in Sorghum using a transformed copy of maizeMs26.
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Wheat ms5 male-sterility is induced by recessive homoeologous A and D genome non-specific lipid transfer proteins.
M. Pallotta,Patricia Warner,Allan Kouidri,Elise J. Tucker,Mathieu Baes,Radoslaw Suchecki,Nathan S. Watson-Haigh,Takashi Okada,Melissa Garcia,Ajay Sandhu,Manjit Singh,Petra Wolters,Marc C. Albertsen,A. Mark Cigan,Ute Baumann,Ryan Whitford +15 more
TL;DR: This work provides genetic information, resources and tools required for successful implementation of ms5 sterility in breeding systems for bread and durum wheats by identifying TaMs5 as a glycosylphosphatidylinositol-anchored lipid transfer protein required for normal pollen exine development, and demonstrating that Ta Ms5-A restores fertility to ms5.
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Patent
Wheat ms1 polynucleotides, polypeptides, and mehtods of use
Marc C. Albertsen,Ute Baumann,Andrew Mark Cigan,Manjit Singh,Elise Tucker,Ryan Whitford +5 more
- 21 Sep 2015
TL;DR: In this article, the level and/or activity of a polynucleotide that influences male fertility in a plant or plant part is modulated in a male-tissue-preferred manner.
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