Mani Subramanian
University of Iowa
19 Papers
155 Citations
Mani Subramanian is an academic researcher from University of Iowa. The author has contributed to research in topics: Theobromine & Caffeine. The author has an hindex of 12, co-authored 19 publications.
Chat about Author
Papers
Two Distinct Pathways for Metabolism of Theophylline and Caffeine Are Coexpressed in Pseudomonas putida CBB5
TL;DR: To the authors' knowledge, this is the first report of theophylline N demethylation and coexpression of distinct pathways for caffeine and theophyLLine degradation in bacteria.
105
Novel, Highly Specific N-Demethylases Enable Bacteria To Live on Caffeine and Related Purine Alkaloids
TL;DR: This work reports the first report of bacterial N-demethylase genes that enable bacteria to live on caffeine and represent a new class of Rieske oxygenases and have the potential to produce biofuels, animal feed, and pharmaceuticals from coffee and tea waste.
99
A Novel Caffeine Dehydrogenase in Pseudomonas sp. Strain CBB1 Oxidizes Caffeine to Trimethyluric Acid
TL;DR: A unique heterotrimeric caffeine dehydrogenase was purified from Pseudomonas sp.
58
Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source.
TL;DR: Ndm was deduced to be a Rieske [2Fe-2S]-domain-containing non-haem iron oxygenase based on its distinct absorption spectrum and significant identity of the N-terminal sequences of NdmA and NdmB with the gene product of an uncharacterized caffeine demethylase in P. putida IF-3 and a hypothetical protein in Janthinobacterium sp.
57
Delineation of the Caffeine C-8 Oxidation Pathway in Pseudomonas sp. Strain CBB1 via Characterization of a New Trimethyluric Acid Monooxygenase and Genes Involved in Trimethyluric Acid Metabolism
TL;DR: Detailed characterization of the second enzyme is reported, a novel NADH-dependent trimethyluric acid monooxygenase (TmuM), a flavoprotein that catalyzes the conversion of TMU to 1,3,7-trimethyl-5-hydroxyisourate (TM-HIU) and S-(+)-TMA, which exhibits no activity on uric acid.
38