https://onlinelibrary.wiley.com/doi/pdf/10.1111/1471-0307.12555

Antimicrobial and antidiabetic potential of synbiotic fermented milk: A functional dairy product

Antioxidant peptides are currently the focus of many studies, since they eliminate free radicals in the human body without harmful effects. In the present study, Lactobacillus casei L61 was used as a starter culture to ferment goat milk because of its high capacity to produce antioxidant peptides. An optimal nutrients formula (casein, casein peptone, glucose, soybean peptone, inulin, calcium lactate, and cysteine) was investigated by Plackett–Burman (P–B) and Box–Behnken (B–B) designs for response surface methodology (RSM). Antioxidant peptides were successively isolated and purified from the fermented goat milk. Furthermore, the stability of the antioxidant peptides was evaluated in a simulated gastrointestinal tract at 37 °C. The results showed that calcium lactate, glucose, and casein peptone significantly affected the antioxidant activity of goat milk. The optimal additive amounts were 0.99% (w/v) calcium lactate, 0.21% (w/v) glucose, and 0.29% (w/v) casein peptone. The hydroxyl free radical scavenging rate increased significantly (p < 0.001) from 56.50 ± 0.57% to 88.01 ± 0.69%; the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate increased up to 63.48 ± 1.22% under the optimal conditions (n = 3). Our research provides a fitted mathematical model for antioxidant peptides production. Besides, these antioxidant peptides had great stability during simulated gastrointestinal digestion.

Antioxidant Peptides from Goat Milk Fermented by Lactobacillus casei L61: Preparation, Optimization, and Stability Evaluation in Simulated Gastrointestinal Fluid

Freeze drying has been well applied in the preparation of high-efficiency probiotic powders. However, the process is generally accompanied by probiotic viability deficiency, which is the bottleneck for further application. To improve the viability of Bifidobacterium bifidum BB01 during freeze-drying, we optimized the cryoprotectant of B. bifidum BB01 by response surface methodology (RSM) with a Central Composite Design (CCD). In this study, two values of B. bifidum BB01 with different protectant factors were investigated, including freeze-drying survival rate and the viable counts of per unit weight of freeze-dried powder. The optimized cryoprotectants were obtained as follows: glycine of 5.5%, sodium bicarbonate of 0.8%, xylo-oligosaccharides of 7%, arginine of 4.5% and skim milk of 25%. The survival rate and the viable counts of per unit weight of powder were 90.37 ± 1.9% and (2.78 ± 0.13) × 1011cfu·g-1, respectively, both close to the predicted value (88.58% and 2.71 × 1011 cfu·g-1). Our research demonstrated that RSM was successful in optimizing composite cryoprotectant for freeze-dried powder of B. bifidum which can as well protect the probiotic cells.

Optimization of composite cryoprotectant for freeze-drying Bifidobacterium bifidum BB01 by response surface methodology.

The present invention relates to a cryoprotecting agent comprising a cryprotectant being one or more of: dextrin, dextran, isomaltooligosaccharide and derivatives thereof, cryoprotecting and cryopreserved compositions, uses thereof, and methods of cryopreservation.

Cryoprotecting agent, cryoprotecting and cryopreserved compositions, uses thereof, and methods of cryopreservation

Prebiotics as Protectants of Lactic Acid Bacteria

Four different prebiotics including fructo-oligosaccharide isomalto-oligosaccharide, inulin and xylo-oligosaccharide were screened for their effects on survival of Bifidobacterium bifidum during freeze-drying. The additions of prebiotics (v/v) were all 4%, 8%, 12%, 16% and 20%. The results showed that prebiotics could act on Bifidobacterium Bifidum. The optimum concentration of fructo-oligosaccharides (FOS) in samples for surival of Bifidobacterium bifidum was 12% and for viable count was 20％. Addition of xylo-oligosaccharides (XOS) played a significant role in promoting cell viablity when it was in low level. The optimum concentration of isomalto-oligosaccharides (IMO) for surival of Bifidobacterium bifidum was 16% and for viable count was 12％, while inulin in samples for surival and viable cell of Bifidobacterium bifidum both was 4％.

Effect of Fructo-Oligosaccharide, Isomalto-Oligosaccharide, Inulin and Xylo-Oligosaccharide on Survival of B. Bifidum during Freeze-Drying

Bifidobacterium bifidum lyophilization composite protection agent and application method thereof

Abstract The objective of the present study was to obtain the optimum proportion of the carbon source and prebiotics for Bifidobacterium bifidum BB01 by the central composite design (CCD). The effect of carbon source (lactose) and two prebiotics (inulin and fructooligosaccharides) on the BB01 were observed by measuring the OD600 value, pH value and the viable counts at 18h. The final optimized concentrations of carbon source and prebiotics were: lactose 1.6%, inulin 0.26%, and fructooligosaccharides 0.22%. The result indicates that the growth of B. bifidum BB01 shows an significant increase in the optimized culture medium (p < 0.05), the OD600 value reached 1.434 at 18h, which increased 6.58% compared to the control. And the viable counts of B. bifidum BB01 increased 24.36% and reached (2.17±0.06) ×109cfu/mL. The results show that the optimization of the carbon source and prebiotics using CCD in this study is workable and necessary.

/pdf/application-of-central-composite-design-to-optimize-the-4xrfdxxdkv.pdf

Application of central composite design to optimize the amount of carbon source and prebiotics for Bifidobacterium bifidum BB01

Abstract In order to improve the viable counts of Bifidobacterium bifidum BB01 in the liquid medium, the Central Composite Design (CCD) was used to optimize the nitrogen source in the medium of B. bifidum BB01. The results showed that the nitrogen source composition of B. bifidum BB01 was: peptone 0.9%, yeast extracts 0.3%, beef paste 0.7%. Under the optimal conditions, the viable counts of B. bifidum BB01 reached (2.49±0.06)×109CFU/mL after cultured at 18h, which was 42.97% higher than MRS (lactose), and 12.85% higher than the optimized MRS medium (carbon source and prebiotics were optimized). Therefore, the CCD used in this study is workable for promoting the growth of B. bifidum BB01.

/pdf/optimization-of-nitrogen-source-for-bifidobacterium-bifidum-38pioekkt2.pdf

Optimization of nitrogen source for Bifidobacterium bifidum using response surface methodology

In order to investigative the effect of different neutralizers and their feeding time on culture of Bifidobacterium bifidum BB01 , and pH, OD and viable count of B. bifidumBB01 in the medium in different time were measured. The results indicated that the NaOH solution was the optimum neutralizer compared with the others, and fe eding time of the neutralizer to B. bifidum BB01 was 13 hours after inoculation. Furthermore, the OD value and viable count reached maximum at 21h (OD value= 1.667) and 20h (viable count: (3.52±0.046) ×10 9 CFU/mL) after the NaOH solution was added to the m edium, respectively. In addition, the maximum OD value implied that the logarithmic phase of B. bifidum BB01 was delayed compared with the control and the viable count were 29.26% larger than the control group. The result of the study provides a method and an important basis for improving the viable counts of B. bifidum BB01 .

https://sciendo.com/pdf/10.1515/aucft-2015-0002

The Effect of Feeding Neutralizer on the Growth of Bifidobacterium Bifidum

Handle everyday research tasks with reliable, citation-backed results

Your personal Research Agent to handle research tasks with citation-backed results

Popular Tasks used by Researchers

How can I help with your research?

Meet SciSpace

Get more enhanced response by uploading the PDFs you want me to reference.

No relevant PDFs in your library

SciSpace is the AI research assistant for academics. Run systematic literature reviews on 280M+ papers, and write papers with cited sources. Trusted by 1M+ students, PhDs & researchers.

SciSpace | AI for Research

Analyze PDFs

Code & Manuscripts

Funding & Grants

Literature & Patents

Medical & Clinical Data

Systematic Review

Visualize & Present

Web & Data

Build a Google Scholar-like website for your research.

Build a website

Create charts and images for your research

Create a Chart

Write a paper for submission to a journal

Draft a manuscript

Patent Search

Design eye-catching scientific posters in minutes.

Scientific Poster Generation

Systematic Literature Review

One task is running at the moment. Your messages will be shown right after.

Drag and drop or click here to browse

Loved by <highlight>1 million+</highlight> researchers

Extract a list of specific topics and their sources from unstructured text

Topics

Compare and analyze relevant papers that matches with your search

Papers

Get insights from PDFs and bookmarked papers from your library

My library

Recent searches

Try searching for:

Catch AI-generated content in scholarly and non-scholarly content

{ai} Detector

Ai Writer

Get PDF Summaries, highlighted text explanations 

Chat with PDF

Effortlessly create in-text citations and bibliographies in APA and 2,500 other formats

Citation generator

Get explanations, summaries, and answers on academic papers

Ease up your research workflow with {scispace}'s cohort of exciting AI tools

Elevate your academic writing skills and convey your ideas the way you want

Paraphraser

Explore our range of reading and writing tools

Your file is being prepared and should be ready in a few minutes. If it's a large file, it might take a bit longer. You can close this window, and we'll email you the file when it's done.

You have reached a maximum limit of <strong>{limit}</strong> columns in the table. Remove at least <strong>1</strong> column to add or create another one.

Man Hu

Author Tools

Chat about Author