Apolipoprotein B 3' (3' ApoB) minisatellite polymorphism was studied in healthy unrelated individuals from the Russian Federation and the Republic of Belarus, in 10 populations from five ethnic groups: Russians, Byelorussians, Adygeis, Kalmyks and Yakuts. The analysis was carried out using PCR and electrophoresis followed by silver staining. Overall, 25 alleles of the 3' ApoB minisatellite, ranging from 25 to 55 repeats, were detected. Heterozygosity indices were high and varied from 0.73 to 0.84. The distributions of alleles of this minisatellite in the Caucasoid populations (Russians, Byelorussians and Adygeis) had a bimodal character, whereas that for Mongoloid populations (Kalmyks and Yakuts) had a unimodal distribution. Nei's genetic distances between the populations studied and some reference populations of Europe and Asia were estimated. Despite their allele distribution homogeneity, different East Slavonic ethnic groups were clearly resolved by multidimensional analyses. The East Slavonic and Adygei populations revealed a high similarity with European Caucasoids. The Mongoloid populations (Kalmyks and Yakuts) were considerably different from those of the European Caucasoid populations, but were similar to other Asian Mongoloid populations. The results demonstrate the variability of 3' ApoB minisatellite polymorphism not only in distant populations but also, to a certain extent, in genetically relative ones.

/pdf/apolipoprotein-b-3-vntr-polymorphism-in-eastern-european-1mc0vdkz22.pdf

Apolipoprotein B 3′-VNTR polymorphism in Eastern European populations

Population data for 12 Y-chromosome STRs in a sample from Brescia (northern Italy).

Genomic DNA was extracted from blood samples of 91 unrelated Uruguayan males that were initially spotted on FTAR paper (Whatman, Clifton, NJ). The six loci (DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385) were amplified using the YPLEXTM6 kit (Reliagene Technologies, Inc., New Orleans, LA) according to the manufacturer’s recommendations and to published experiences (1). The amplified products were analysed by capillary electrophoresis using the ABI Prism 310 DNA Sequencer (PE Biosystems, Foster City, CA). Allele assignment was performed by comparison with self-established allelic ladder, positive and negative controls included in the kit. Several external populations were used for comparison from previously published papers; these are samples from Southern Spain and the Spanish Canary Islands (2), Northern Argentinian Amerindians (3), Italians (4), Mozambique (5), Caucasian Argentinians (6), Brazilians (5), and from Portugal (7). Since duplicated loci such as DYS385 cannot be dealt with in AMOVA, the study had to be confined to the systems DYS393, DYS19, DYS389II, DYS390, DYS391. STR allele frequencies, diverse Y-chromosome diversity indices and population differentiation were tested using software ARLEQUIN version 2.000 (8). The allele frequencies and gene diversity of the STR’s DYS393, DYS19, DYS389II, DYS390, DYS391 y DYS385 from Uruguay are shown in Table 1. We counted 76 different haplotypes in a population sample of 91 Uruguayan males giving a haplotype diversity

A genetic population study of six Y STRS (Y-Plex6) in an Uruguayan sample and its comparison with other populations.

Distribution of HumvWA31, HumFESFPS, HumTH01, HumTPOX, HumCD4, HumCSF1PO Alleles in a Southern Italian Population Sample

Distribution of HumvWA31, HumFESFPS, HumTH01, HumTPOX, HumCD4, HumCSF1PO alleles in a Southern Italian population sample.

The object of this work was to examine a set of Y-STR systems in the population of Brescia (North Italy) to create a database. In the present study, 51 DNA samples taken from unrelated males were analysed and PCR amplification of DYS19, DYS389-I/II, DYS390, DYS391, DYS392 and DYS393 was performed. PCR products were separated by manual procedures. The frequencies of Y-STR haplotypes are presented.

A genetic population study of seven Y-chromosome STR-loci in a population of Brescia area (North Italy)

Population study and paternity testing of seven Y-chromosome STR-loci in an italian population sample

HUMTH01 polymorphism, first described by Edwards et al (1991), is due to a variation in the length of a tetrameric short tandem repeat on locus 11p15.5. Seven alleles were identified in the range 183–207 bp (Edwards et al 1992).

The Distribution of HUMTH01 Polymorphism in Northern and Central Italy

The tetrameric (ATTT) short tandem repeat HUMFES/FPS system, located on chromosome 15 (15q25-qter), displays a polymorphism (Polymeropoulos et al. 1991) which has been investigated for forensic purposes (Hammond et al. 1994). The aim of the present work was to study the polymorphism of this system in a large Italian population sample: 1) to verify allele frequency distribution, 2) to check whether allele frequencies show inter-regional differences, 3) to evaluate the effectiveness of this system for paternity testing and personal identification. For this purpose, collaborative research was performed on subjects from four Italian regions in laboratories of the Institutes of Legal Medicine of the Universities of Pavia (Lombardy), Parma (Emilia), Pisa (Tuscany) and Ancona (Marches).

Allele Frequencies of the HUMFES/FPS System in Northern and Central Italy

The APO B VNTR locus is located next to the 3′ end of the apolipoprotein B gene and consists of a series of AT rich tandem repeats, each 14–16 bp long (for a review about the genetic relationship between the 3′-VNTR and diallelic apolipoprotein B gene polymorphism, see Renges et al, 1992; for a recent proposal of a molecular model, see Desmarais et al 1993) Its polymorphism was described by Boerwinkle et al (1989) and by Ludwig et al (1989) using the PCR technique Since then, the APO B has become a genetic marker quite frequently used in forensic practice

Study of the APO B Polymorphism in Tuscany (Italy)

RFLP (restriction fragment-lenght polymorphism) at the D2S44 locus, detected by the highly polymorphic probe YNH24 (Nakamura et al. 1987), can be used as an efficient tool in stain and paternity testing, as long as reliable fragment size frequencies are available (see van Eede et al. 1991, Gill et al. 1991, Budowle et al. 1991).

Distribution of variable number of tandem repeat (VNTR) DNA polymorphism at D2S44 locus in Tuscany (Italy)

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