M. Doi
University of Tokyo
6 Papers
74 Citations
M. Doi is an academic researcher from University of Tokyo. The author has contributed to research in topics: Gene & Peptide sequence. The author has an hindex of 6, co-authored 6 publications.
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Papers
Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus.
Michio Matsuhashi,Min Dong Song,Fumitoshi Ishino,Masaaki Wachi,M. Doi,M. Inoue,Kimiko Ubukata,N Yamashita,Masatoshi Konno +8 more
TL;DR: A novel penicillin-binding protein, PBP-2' (Mr about 75,000), is known to be induced in excessively large amount by most beta-lactam compounds in cells of a clinically isolated strain of Staphylococcus aureus, TK784, that is highly resistant to beta- lactams and also most other antibiotics.
348
Mutant isolation and molecular cloning of mre genes, which determine cell shape, sensitivity to mecillinam, and amount of penicillin-binding proteins in Escherichia coli.
TL;DR: A chromosomal region of Escherichia coli contiguous to the fabE gene at 71 min on the chromosomal map contains multiple genes that are responsible for determination of the rod shape and sensitivity to the amidinopenicillin mecillinam.
255
Determinations of the DNA sequence of the mreB gene and of the gene products of the mre region that function in formation of the rod shape of Escherichia coli cells.
M. Doi,Masaaki Wachi,Fumitoshi Ishino,Shigeo Tomioka,Michihiko Ito,Y Sakagami,A Suzuki,Michio Matsuhashi +7 more
TL;DR: The 6.5-kilobase mre region at 71 min in the Escherichia coli chromosome map, where genes involved in formation of a rod-shaped cell form a gene cluster, was analyzed by in vivo protein synthesis in a maxicell system and by base sequencing of DNA.
192
Genes encoding two lipoproteins in the leuS-dacA region of the Escherichia coli chromosome.
I Takase,Fumitoshi Ishino,Masaaki Wachi,H Kamata,M. Doi,S Asoh,Hiroshi Matsuzawa,Takahisa Ohta,Michio Matsuhashi +8 more
TL;DR: The coding of two rare lipoproteins by two genes, rlpA and rlpB, located in the leuS-dacA region (15 min) on the Escherichia coli chromosome was demonstrated by expression of subcloned genes in a maxicell system.
77
New mre genes mreC and mreD, responsible for formation of the rod shape of Escherichia coli cells.
TL;DR: New shape-determining genes in the mre cluster at 71 min on the Escherichia coli chromosome map, named mreC and mreD, were identified by complementation experiments using delta mre-678 mutant cells, and by DNA sequencing.