Lukas Frank

TL;DR: In this paper, the authors dissected the mechanism of transcription activation at a reporter gene array with real-time single-cell fluorescence microscopy readouts and found that the phase separation propensity of the activation domain correlates with high transcription activation capacity by increasing binding site occupancy, residence time and the recruitment of co-activators.

TL;DR: In this article, the authors dissected transcription activation by comparing different synthetic TFs at a reporter gene array with real-time single-cell fluorescence microscopy readouts, and concluded that multivalent AD mediated interactions increase the transcription activation capacity of a TF by stabilizing chromatin binding and mediating the recruitment of co-activators independent of phase separation.

TL;DR: In this paper, the authors describe how to activate pericentric heterochromatin (PCH) in mouse cells as a prototypic silenced state by recruiting fusions of the catalytically inactive dCas9 protein in a sequence-specific manner to a locus of interest.

TL;DR: The findings reveal that adult hippocampal MeCP2 is required to finetune alternative splicing events in basal conditions, as well as in response to spatial learning, and sheds light onto the pathophysiological mechanisms of Rett syndrome.