Luisa Roller
University of Tübingen
2 Papers
19 Citations
Luisa Roller is an academic researcher from University of Tübingen. The author has contributed to research in topics: Gene & Heterologous expression. The author has an hindex of 2, co-authored 2 publications.
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Papers
The Immunosuppressant Brasilicardin: Determination of the Biosynthetic Gene Cluster in the Heterologous Host Amycolatopsis japonicum.
Paul N. Schwarz,Anina Buchmann,Luisa Roller,Andreas Kulik,Harald Gross,Wolfgang Wohlleben,Evi Stegmann +6 more
TL;DR: Investigation of the genes flanking the previously defined brasilicardin biosynthetic gene cluster revealed two novel genes (bra0, bra12), which are involved in brasilicARDin biosynthesis: bra12 encodes a transcriptional activator of the brasicardin gene cluster and bra0 codes for a dioxygenase involved in methoxylation of brasilicards.
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Engineering metabolic pathways in Amycolatopsis japonicum for the optimization of the precursor supply for heterologous brasilicardin congeners production.
TL;DR: Simultaneous heterologous expression of genes encoding the MVA pathway and expression of diterpenoid specific prenyltransferases contributed to an elevated heterOLOGous production of the brasilicardin backbone, which can now be used as a starting point for semi synthesis of new brasilicARDin congeners with better properties.
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