Luís P. Fonseca
Instituto Superior Técnico
171 Papers
1.1K Citations
Luís P. Fonseca is an academic researcher from Instituto Superior Técnico. The author has contributed to research in topics: Cutinase & Chemistry. The author has an hindex of 32, co-authored 163 publications. Previous affiliations of Luís P. Fonseca include Technical University of Lisbon & Centro Hospitalar de Vila Nova de Gaia/Espinho.
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Papers
Immobilization studies of an industrial penicillin acylase preparation on a silica carrier.
TL;DR: High immobilization yields, operational stability, enzyme loading and support reutilization capacity were obtained, and the operational stabilities of penicillin acylase coupled covalently to the silica gel support and immobilized by a crosslinking procedure using glutaraldehyde were compared.
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Biosynthesis of ethyl caproate and other short ethyl esters catalyzed by cutinase in organic solvent
Dragana P.C. de Barros,Luís P. Fonseca,Pedro Fernandes,Joaquim M. S. Cabral,Ljiljana Mojović +4 more
TL;DR: In this article, Fusarium solani pisi cutinase was used for short chain carboxylic acid (C 4 -C 6 ) ester synthesis in iso-octane.
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Production of cellulases in batch culture using a mutant strain of Trichoderma reesei growing on soluble carbon source
TL;DR: This strain of T. reesei Rut-C30 is a highly derepressed mutant which synthesised active cellulases in culture media containing glucose and lactose as the only carbon sources and had a higher specific filter paper activity than was produced by other T.Reesei mutants.
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Variation of penicillin acylase partition coefficient with phase volume ratio in poly(ethylene glycol)-sodium citrate aqueous two-phase systems
TL;DR: The influence of phase volume ratio on partition and purification of penicillin acylase from Escherichia coli on poly(ethylene glycol)-sodium citrate aqueous two-phase systems was studied and implications for protein purification designs are discussed.
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Production and characterization of recombinant cyprosin B in Saccharomyces cerevisiae (W303-1A) strain
Pedro N. Sampaio,Ana Margarida Fortes,Joaquim M. S. Cabral,Maria Salomé Pais,Luís P. Fonseca +4 more
TL;DR: Rec recombinant cyprosin B, the first clotting protease of plant origin produced in a bioreactor, can now be produced in large scale and may constitute a new and efficient alternative to enzymes of animal or fungal origin that are widely used in cheese making.
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