One-step microwave-assisted synthesis and characterization of novel CuO nanodisks for non-enzymatic glucose sensing

Quantitative comparison of iron and cobalt based catalysts for the Fischer-Tropsch synthesis under clean and poisoning conditions

Electrospinning of highly dispersed Ni/CoO carbon nanofiber and its application in glucose electrochemical sensor

A highly sensitive and selective method for determination of phenoxy carboxylic acids from environmental water samples by dispersive solid-phase extraction coupled with ultra high performance liquid chromatography-tandem mass spectrometry.

Photoelectrodes constructed with photoactive materials are of great significance for the performance of photoelectrochemical (PEC) sensors. In this study, a facile and efficacious non-enzymatic glucose sensor based on the Cu 2 O/TiO 2 heterojunction was successfully constructed for quantitative determination of glucose concentration. The Cu 2 O/TiO 2 heterojunction was generated by first obtaining TiO 2 nanofibers via electrostatic spinning and high-temperature calcination, and then synthesizing Cu 2 O nanoparticles via a one-step hydrothermal reaction. By introducing the narrow band gap semiconductor Cu 2 O, the defect that pure TiO 2 could not absorb visible light was improved, resulting in the improved photoelectrochemical performance of the composite material compared to the single component semiconductor. The sensor exhibited favorable PEC non-enzymatic glucose sensing performance under simulated sunlight irradiation with a wide linear response range from 1 mM to 14 mM (R 2 = 0.9991) and a low detection limit of 14.4 μM (S/N = 3), along with good selectivity, stability and reproducibility. This also provided more ideas and possibilities for preparing photoelectrode materials by constructing heterojunctions. 

Construction of Cu2O/TiO2 heterojunction photoelectrodes for photoelectrochemical determination of glucose

Quantification of endogenous and exogenous plasma glucose can help more comprehensively evaluate the glucose metabolic status. A ratio-based approach using isotope dilution liquid chromatography tandem mass spectrometry (ID LC-MS/MS) with indirect multiple reaction monitoring (MRM) of the derivative tag was developed to simultaneously quantify endo-/exogenous plasma glucose. Using diluted D-[13C6] glucose as tracer of exogenous glucose, 12C6/13C6 glucoses were first derivatized and then data were acquired in MRM mode. The metabolism of exogenous glucose can be tracked and the concentration ratio of endo/exo-genous glucose can be measured by calculating the endo-/exo-genous glucose concentrations from peak area ratio of specific daughter ions. Joint application of selective derivatization and MRM analysis not only improves the sensitivity but also minimizes the interference from the background of plasma, which warrants the accuracy and reproducibility. Good agreement between the theoretical and calculated concentration ratios was obtained with a linear correlation coefficient (R) of 0.9969 in the range of D-glucose from 0.5 to 20.0 mM, which covers the healthy and diabetic physiological scenarios. Satisfactory reproducibility was obtained by evaluation of the intra- and inter-day precisions with relative standard deviations (RSDs) less than 5.16%, and relative recoveries of 85.96 to 95.92% were obtained at low, medium, and high concentration, respectively. The method was successfully applied to simultaneous determination of the endo-/exogenous glucose concentration in plasma of non-diabetic and type II diabetic cynomolgus monkeys.

Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag.

It has been a challenging task of analytical chemistry to qualitatively and quantitatively analyze the diverse compounds in the complex mixture. Liquid chromatography-mass spectroscopy (LC-MS), especially, ultra-high performance liquid chromatography (UPLC) coupled with high resolution mass spectrometry (HRMS), is a powerful tool for the analysis of complex samples. UPLC can greatly enhance the ability and efficiency of chromatographic separation. On the other hand, HRMS can provide more precise molecular weight and can thus improve the reliability and accuracy of qualitative and quantitative analysis. In this paper, we summarized the application of LC-MS technology to analysis of small molecule compounds in typical complex mixtures (urine, blood, cell metabolites and natural product extracts), concentrating on sample preparation, data acquisition, data (pre)processing, qualitative and quantitative analysis, multivariate analysis. Furthermore, technical challenges and developments in the analysis of complex samples by LC-MS were summarized and prospected.

/pdf/analysis-of-small-molecule-compounds-in-complex-mixture-45b20cpcko.pdf

Analysis of small molecule compounds in complex mixture system by LC-MS

A wide variety of methods, such as enzymatic methods, LC-MS, and LC-MS/MS, are currently available for the concentration determination of plasma glucose in studies of diabetes, obesity, exercise, etc However, these methods rarely discriminate endogenous and exogenous glucose in plasma A novel NMR strategy for discriminative quantification of the endogenous and exogenous glucose in plasma has been developed using an adapted isotope dilution 1H–13C heteronuclear single-quantum correlation (ID-HSQC) with uniformly 13C-labeled glucose as a tracer of exogenous glucose This method takes advantage of the distinct 1H–13C chemical shifts of the hemiacetal group of the α-D-glucopyranose and makes use of the 13C–13C one-bond J-coupling (1JCC) in uniformly 13C-labeled glucose to differentiate the 1H–13C HSQC signal of labeled glucose from that of its natural counterpart when data are acquired in high-resolution mode The molar ratio between the endogenous and exogenous plasma glucose can then be calculated from the peak intensities of the natural and labeled glucose The accuracy and precision of the method were evaluated using a series of standard mixtures of natural and uniformly 13C-labeled glucose with varied but known concentrations Application of this method is demonstrated for the quantification of endogenous and exogenous glucose in plasma derived from healthy and diabetic cynomolgus monkeys The results nicely agree with our previous LC-MS/MS results Considering the natural abundance of 13C isotope at the level of 11% in endogenous glucose, comparable peak intensities of quantitatively measurable signals derived from natural and labeled glucose imply that the ID-HSQC can tolerate a significantly high ratio of isotope dilution, with labeled/natural glucose at ~ 1% We expect that the ID-HSQC method can serve as an alternative approach to the biomedical or clinical studies of glucose metabolism

An adapted isotope dilution 1 H– 13 C heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose

The invention provides a two-dimensional nuclear magnetic resonance method for simultaneously quantifying endogenous glucose and exogenous glucose in plasma. according to the two-dimensional nuclear magnetic resonance method, by utilizing an isotope dilution method taking a small amount of D-[U- C] glucose as a tracer and an internal standard and combining an adaptively-adapted nuclear magnetism singular quantum correlation experiment (ID-HSQC), and the corresponding ratio of the dual-peak integral to the single-peak integral is determinated so as to calculate the concentration of the endogenous glucose and the exogenous glucose in the plasma. The method has the advantages that a sample is simple to prepare (pretreatment steps such as deproteinization and derivatization are not needed),the usage amount of the isotope labelling tracer is small, the concentration of the endogenous glucose and the exogenous glucose in the plasma can be measured simultaneously and accurately, and the like.

Two-dimensional nuclear magnetic resonance method for simultaneously quantifying endogenous glucose and exogenous glucose in plasma

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Simultaneous quantification of endogenous and exogenous plasma glucose by isotope dilution LC-MS/MS with indirect MRM of the derivative tag.

Analysis of small molecule compounds in complex mixture system by LC-MS

An adapted isotope dilution 1 H– 13 C heteronuclear single-quantum correlation (ID-HSQC) for rapid and accurate quantification of endogenous and exogenous plasma glucose

Two-dimensional nuclear magnetic resonance method for simultaneously quantifying endogenous glucose and exogenous glucose in plasma