Blackberries are an emerging third-generation fruit that are popular in Europe, and specific nitrogen (N) supply is an important factor affecting their growth and development. To study the optimal N fertilizer for blackberry seedlings, no N (CK), nitrate (NO3−)–N, ammonium (NH4+)–N and urea were applied to one-year-old ‘Ningzhi 4’ blackberry plants at a key growth period (from May to August) to explore the effects of different N forms on the physiological characteristics. Correlation and principal component analysis were used to determine the relationships between various indexes. Ammonium (NH4+) or urea-fed plants had a better growth state, showed a greater plant height, biomass, SPAD values and enhanced antioxidant enzyme activities and photosynthesis. In addition, NH4+ was beneficial to the accumulation of sugars and amino acids in leaves and roots, and promoted the transport of auxin and cytokinin to leaves. NO3− significantly inhibited root growth and increased the contents of active oxygen, malondialdehyde and antioxidants in roots. Correlation and principal component analysis showed that growth and dry matter accumulation were closely related to the antioxidant system, photosynthetic characteristics, amino acids and hormone content. Our study provides a new idea for N regulation mechanism of blackberry and proposes a scientific fertilization strategy.

/pdf/physiological-and-morphological-responses-of-blackberry-1d9m1xfv.pdf

Physiological and Morphological Responses of Blackberry Seedlings to Different Nitrogen Forms

Soil acidification in Chinese tea plantations is widespread, and it has significantly affected the growth of tea trees; it was important to explore soil remediation of acidified tea plantations in depth for the sustainable development of tea industry. In this study, the effects of sheep manure fertilizer with different application depths on soil acidification, tea yield and quality, and soil nitrogen transformation in tea plantations were analyzed for five consecutive years from 2018 to 2022. The results showed that long-term use of sheep manure fertilizer significantly reduced soil acidification (P< 0.05) in tea plantations, improved soil pH and soil ammonium nitrogen content, enhanced root activity and root nitrogen uptake capacity of tea trees, and thus improved tea yield and quality. The effect of different application depths of sheep manure fertilizer on tea yield and quality was mainly reflected in the transformation ability of soil ammonium nitrogen and nitrate nitrogen, which showed that high transformation ability of soil ammonium nitrogen and high ammonium nitrogen content were beneficial to high tea yield and vice versa, and the best effect was achieved when sheep manure was applied at a depth of 50 cm and 70 cm. The topsis analysis confirmed that sheep manure fertilization had a greater effect on root activity, ammonium nitrogen, ammonia intensity, and nifH gene. This study provided an important practical basis for the restoration of acidified tea plantation soil through sheep manure fertilizer management.

https://www.frontiersin.org/articles/10.3389/fpls.2023.1179960/pdf

Reasonable deep application of sheep manure fertilizer to alleviate soil acidification to improve tea yield and quality

Panax notoginseng is a precious Chinese medicinal material. Soil fumigation can control soil-borne disease and overcome the continuous cropping obstacles of P. notoginseng. However, chloropicrin (CP) fumigation can kill non-target soil microorganisms and reduce microbial diversity, but the long-time impacts of CP fumigation on soil microbial are less reported.We studied the long-term effects of CP fumigation on soil microbes with high-throughput gene sequencing, and correlated the changes in the composition of microbial communities with environmental factors like soil physicochemical properties and soil enzyme activities. This study mainly focuses on the recovery characteristics of soil microbe after soil fumigation by evaluating the ecological restoration of P. notoginseng soil, its sustained control effect on plant diseases, and its promotion effect on crop growth by focusing on the CP fumigation treatment.The results showed that CP fumigation significantly increased soil available phosphorus (P) to 34.6 ~ 101.6 mg/kg and electrical conductivity (EC) by 18.7% ~ 34.1%, respectively. High-throughput gene sequencing showed that soil fumigation with CP altered the relative abundance of Trichoderma, Chaetomium, Proteobacteria, and Chloroflexi in the soil while inhibiting a lot of Fusarium and Phytophthora. The inhibition rate of Phytophthora spp. was still 75.0% in the third year after fumigation. Fumigation with CP enhanced P. notoginseng’s survival rate and stimulated plant growth, ensuring P. notoginseng’s healthy in the growth period. The impact of fumigation on microbial community assembly and changes in microbial ecological niches were characterized using normalized stochasticity ratio (NST) and Levins’ niche breadth index. Stochasticity dominated bacterial community assembly, while the fungal community was initially dominated by stochasticity and later by determinism. Fumigation with CP reduced the ecological niches of both fungi and bacteria.In summary, the decrease in microbial diversity and niche caused by CP fumigation could be recovered over time, and the control of soil pathogens by CP fumigation remained sustainable. Moreover, CP fumigation could overcome continuous cropping obstacles of P. notoginseng and promote the healthy growth of P. notoginseng.

Long-term effects of chloropicrin fumigation on soil microbe recovery and growth promotion of Panax notoginseng

Plants periodically shed more than 90% of their biomass production as above- and below-ground litter, including leaves, twigs, flowers, logs, roots and other tissues [...]

/pdf/carbon-and-nutrient-transfer-via-above-and-below-ground-2hlp11jy.pdf

Carbon and Nutrient Transfer via Above- and Below-Ground Litter in Forests

Pear (Pyrus communis)-based agroecosystem improves soil nutrient dynamics, microbial biomass, enzymatic activity, farm productivity and profitability

Nitrogen fertilization is a simple and effective field management strategy for increasing plant productivity, but the regulatory mechanisms of nitrogen forms and proportions on soil nutrients and plant growth remain unclear. Therefore, we investigated soil enzyme activities and nutrient contents of alfalfa under different forms and proportions of exogenous nitrogen addition. Results showed that nitrogen input significantly increased the activity of three oxidoreductases (hydroxylamine reductase, nitrate reductase, and nitrite reductase) while having no significant effects on urease. A high proportion of ammonium nitrogen significantly increased neutral protease activity. The amylase activity markedly increased under mixed-nitrogen addition but decreased under single-nitrogen addition. Additionally, the contents of soil nutrients (soil organic matter, total nitrogen, nitrate nitrogen, ammonium nitrogen, available phosphorus, and available potassium) were significantly increased under different exogenous nitrogen inputs, which drove the changes in enzyme activities. Further, nitrogen addition also improved the biomass and nitrogen content of alfalfa. These findings indicated that applying different forms and proportions of exogenous nitrogen may stimulate soil enzyme activities, which will accelerate the transformation of nutrients and then promote alfalfa growth.

/pdf/different-forms-and-proportions-of-exogenous-nitrogen-3ab9zgo6.pdf

Different Forms and Proportions of Exogenous Nitrogen Promote the Growth of Alfalfa by Increasing Soil Enzyme Activity

The differences between aquatic and terrestrial habitats could change microbial community composition and regulate litter decomposition in a subtropical forest, but the linkage remains uncertain. Using microbial phospholipid fatty acids (PLFAs), the litter decomposition associated with microbial organisms was monitored to characterize the differences of microbial communities in the forest floor, headwater stream, and intermittent stream. Habitat type did not significantly affect the concentrations of total PLFA. However, microbial community composition (fungi, G+ bacteria, and eukaryote) was significantly affected by the microenvironment among habitats. Compared with which in headwater stream, more individual PLFAs were identified in the natural forest floor and the intermittent stream during the whole decomposition period. The differences in individual PLFA concentrations were reflected in the forest floor and aquatic system in the early stage of litter decomposition, but they mainly reflected in the headwater stream and the intermittent stream in the later stage of litter decomposition. We linked the relationships between microbial community and litter decomposition and found that communities of decomposers drive differences in litter decomposition rate among habitats. Intriguingly, the microbial community showed the greatest correlation with the decomposition rate of litter in streams. These findings could contribute to the understanding of habitats difference on the microbial community during litter decomposition.

/pdf/effects-of-habitat-differences-on-microbial-communities-3eg8iyju.pdf

Effects of Habitat Differences on Microbial Communities during Litter Decomposing in a Subtropical Forest

Enzyme activity plays a pivotal role in leaf litter decomposition, but the variations have not been well addressed in the forest canopy with amounts of leaf litter. Therefore, eight enzymes related to carbon, nitrogen, and phosphorus mineralization were checked during Castanopsis carlesii leaf litter decomposition in the forest canopy and on the forest floor from April 2021 to February 2022. The results displayed that most enzyme activities were lower in the forest canopy compared to the forest floor during litter decomposition, except for acid phosphatase, polyphenol oxidase, and peroxidase activities. Moreover, enzyme stoichiometry and enzyme vector features indicated that the microbes in both habitats were limited by carbon and phosphorus during litter decomposition. Much stronger carbon limitation was detected on the forest floor, while phosphorus limitation was higher in the forest canopy. Phosphorus limitation was weakened, but carbon limitation was strengthened in the forest canopy with leaf litter decomposition. Additionally, the redundancy analysis revealed that air temperature dominated the variations in enzyme activities during litter decomposition in the forest canopy, and litter mass-loss rate in each period explained much more dynamics on the forest floor compared with those in the forest canopy. These results provide new insight into a comprehensive understanding of litter decomposition in subtropical forests.

/pdf/dynamics-of-enzyme-activities-during-the-decomposition-of-657ixyrc.pdf

Dynamics of Enzyme Activities during the Decomposition of Castanopsis carlesii Leaf Litter in the Forest Canopy and Forest Floor in a Mid-Subtropical Area

Changes in acid-hydrolyzable carbohydrates and acid-unhydrolyzable residue during foliar litter decomposition of Castanopsis carlesii and Chinese fir in a subtropical forest headwater stream

To assess the dynamics and spectral characteristics of dissolved organic matter of twig litter in continuous increase stage, peak stage, and continuous decrease stage of twig litter production in different types of Castanopsis carlesii forest in middle subtropical China, a field experiment was conducted in C. carlesii natural forest, secondary forest and plantation. The results showed that litter production stage and forest type significantly affected the content and spectral characteristics of dissolved organic matter of twig litter were . Compared with the secondary forest and plantation, natural forest had higher dissolved organic carbon (DOC) content and lower special ultraviolet-visible absorption values at 254, 260 and 280 nm (SUVA254, SUVA260, SUVA280) at the continuous decrease stage of twig litter production, indicating high twig litter quality of natural forest and high cycling efficiency with dissolved organic matter in the natural forest at this stage. In contrast, the higher contents of total nitrogen (TN), total phosphorus (TP), total dissolved nitrogen (TDN), total dissolved phosphorus (TDP), and lower DOC:TDP and TDN:TDP ratios of twig litter in the plantation were observed at the peak stage of twig litter production, while no differences were detected in dissolved organic matter contents and spectral values in the secondary forest among the stages. In addition, the DOC, TDN, TDP of twig litter were negatively correlated with temperature and precipitation in the natural forests and secondary forests, but TDN and TDP of twig litter were positively correlated with temperature and precipitation in the plantations. These results suggested that the higher nutrient content at the peak stage of twig litter production in the C. carlesii plantation might lead to more efficient material cycling and that there would be a higher efficiency of material cycling for twig litter dissolved organic matter in C. carlesii natural forest at reduction stage of twig litter production.

[Dissolved organic matter dynamics and spectral characteristics of twig litter from different Castanopsis carlesii forests in the middle subtropical region, China].

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