Lindsay Peed
United States Environmental Protection Agency
12 Papers
148 Citations
Lindsay Peed is an academic researcher from United States Environmental Protection Agency. The author has contributed to research in topics: Bacteroidales & Indicator bacteria. The author has an hindex of 9, co-authored 12 publications.
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Papers
Evaluation of genetic markers from the 16S rRNA gene V2 region for use in quantitative detection of selected Bacteroidales species and human fecal waste by qPCR.
Richard A. Haugland,Manju Varma,Mano Sivaganesan,Catherine A. Kelty,Lindsay Peed,Orin C. Shanks +5 more
TL;DR: Analysis for multiple individual Bacteroidales species may be useful in identifying human fecal pollution in environmental waters, as suggested by results of this study.
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Performance of human fecal anaerobe-associated PCR-based assays in a multi-laboratory method evaluation study
Blythe A. Layton,Yiping Cao,Darcy L. Ebentier,Kaitlyn T. Hanley,Elisenda Ballesté,João Brandão,Muruleedhara N. Byappanahalli,Reagan R. Converse,Reagan R. Converse,Andreas H. Farnleitner,Jennifer Gentry-Shields,Maribeth L. Gidley,Michèle Gourmelon,Chang-Soo Lee,Jiyoung Lee,Solen Lozach,Tania Madi,Wim G. Meijer,Rachel T. Noble,Lindsay Peed,Georg H. Reischer,Raquel Rodrigues,Joan B. Rose,Alexander Schriewer,Chris Sinigalliano,Sangeetha Srinivasan,Jill R. Stewart,Laurie C. Van De Werfhorst,Dan Wang,Richard L. Whitman,Stefan Wuertz,Stefan Wuertz,Jenny Jay,Patricia A. Holden,Alexandria B. Boehm,Orin C. Shanks,John F. Griffith +36 more
TL;DR: Overall, HF183 Taqman(®) was found to be the most effective marker of human fecal contamination in this California-based study.
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Decay of bacterial pathogens, fecal indicators, and real-time quantitative PCR genetic markers in manure-amended soils.
Shane Rogers,Matthew Donnelly,Lindsay Peed,Catherine A. Kelty,Sumona Mondal,Zirong Zhong,Orin C. Shanks +6 more
TL;DR: Host-associated qPCR genetic markers for microbial source tracking decayed rapidly to nondetectable concentrations, long before FIB, Salmonella enterica serovar Typhimurium/pDs, and E. coli O157:H7/pZs, suggesting they may not be reliable indicators of nonpoint source fecal contamination events that occur weeks following manure application on land.
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Interlaboratory comparison of real-time PCR protocols for quantification of general fecal indicator bacteria
Orin C. Shanks,Mano Sivaganesan,Lindsay Peed,Catherine A. Kelty,A. Denene Blackwood,Monica R. Greene,Rachel T. Noble,Rebecca N. Bushon,Erin A. Stelzer,Julie L. Kinzelman,Tamara Anan’eva,Christopher D. Sinigalliano,David Wanless,John F. Griffith,Yiping Cao,Steve B. Weisberg,V.J. Harwood,Christopher Staley,Kevin Oshima,Manju Varma,Richard A. Haugland +20 more
TL;DR: Examination of interlaboratory variability in the measurement of enterococci and Bacteroidales concentrations from standardized, spiked, and environmental sources of DNA using the Entero1a and GenBac3qPCR methods suggests that a crude lysate is sufficient for DNA isolation unless environmental samples contain substances that can inhibit qPCR amplification.
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Factors affecting the presence of human-associated and fecal indicator real-time quantitative PCR genetic markers in urban-impacted recreational beaches.
Marirosa Molina,Shayla Hunter,Mike Cyterski,Lindsay Peed,Catherine A. Kelty,Mano Sivaganesan,Thomas Mooney,Lourdes Prieto,Orin C. Shanks +8 more
TL;DR: Both alongshore movement of currents and SD outflow as significant influences on the concentration of molecular and culturable indicators in the bathing zone are identified, suggesting that combining methodologies is a useful and cost effective approach to help understand transport dynamics of fecal contamination and identify potential sources of contamination at marine beaches.
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