Recent advances on protein separation and purification methods.

Nanoparticles (NPs) have remarkable properties for delivering therapeutic drugs to the body’s targeted cells. NPs have shown to be significantly more efficient as drug delivery carriers than micron-sized particles, which are quickly eliminated by the immune system. Biopolymer-based polymeric nanoparticles (PNPs) are colloidal systems composed of either natural or synthetic polymers and can be synthesized by the direct polymerization of monomers (e.g., emulsion polymerization, surfactant-free emulsion polymerization, mini-emulsion polymerization, micro-emulsion polymerization, and microbial polymerization) or by the dispersion of preformed polymers (e.g., nanoprecipitation, emulsification solvent evaporation, emulsification solvent diffusion, and salting-out). The desired characteristics of NPs and their target applications are determining factors in the choice of method used for their production. This review article aims to shed light on the different methods employed for the production of PNPs and to discuss the effect of experimental parameters on the physicochemical properties of PNPs. Thus, this review highlights specific properties of PNPs that can be tailored to be employed as drug carriers, especially in hospitals for point-of-care diagnostics for targeted therapies.

/pdf/exploring-various-techniques-for-the-chemical-and-biological-2auxrk0g.pdf

Exploring Various Techniques for the Chemical and Biological Synthesis of Polymeric Nanoparticles

Sesame seed as an alternative plant protein source: A comprehensive physicochemical characterisation study for alkaline, salt and enzyme-assisted extracted samples

The methods commonly used for separation of the components in an infusion of black tea are chromatographic using silica gel, cellulose and Sephadex as the adsorbents. separation evaluation the quality was to separation of the phenolic components in tea infusion. The methods and results

Separation of the components in black tea infusion by chromatography on Toyopearl [a hydrophilic porous polymer packing material for gel filtration]

In the modern era, nanomedicine has developed novel drug-delivery strategies to improve chemotherapy. Nanotechnological-based treatment approaches for cancer through targeted tumour drug delivery and stimulus-responsive tumour microenvironment have gained tremendous success in oncology. The application of building block materials of these nanomedicines plays a vital role in cancer remediation. Despite successful application in various medical treatments, nanocarriers' lack of biodegradability and biocompatibility makes their use in a clinical context difficult. In addition, the preparation of current drug delivery systems is a major constraint. The current cancer treatment methods aim to destroy diseased tissue, frequently with the use of radiation and chemotherapy. These treatment options are accompanied by a significant level of toxicity, which has excellent potential to further medical issues in the afflicted patient. Polyhydroxyalkanoate (PHA) polymers are biodegradable and biocompatible polyesters that can potentially be used as nanoparticular delivery systems for cancer treatment. Previously, PHA has shown tremendous application as a packaging material in the food and pharma industry. PHA-based nanocarriers are an effective drug delivery system because of their non-immunogenicity, regulated drug release, high drug loading capacity, and targeted drug delivery. This review focuses on creating and using PHA-based nanocarriers in cancer treatment. Despite its many benefits, PHA-based nanocarriers have yet to progress to clinical trials for drug delivery applications due to several issues, including the polymers' hydrophobic nature and high production costs. This review examines these challenges along with existing alternatives.

Polyhydroxyalkanoates (PHAs) and its copolymer nanocarrier application in cancer treatment: An overview and challenges

In the current experiment, the integrated techniques of salting-out extraction followed by cation exchange chromatographic separation were used to purify lactoperoxidase (LP) from milk whey. In order to optimize the purification condition of LP, the different conditions of salting out extraction as well as chromatographic separation were tried and the efficiency of these conditions were evaluated in terms of activity recovery and purification fold. LP extracted with ammonium sulphate for 8 h at pH 6.5, followed by centrifugation at 7000 rpm was revealed as optimum salting condition due to maximum value activity recovery (85.74%) and purification fold (3.13%). LP from this optimum salting out extraction process were furthermore separated in 100 GigaCap S-650M strong cation chromatographic column (20 cm × 1.6 cm, 50–100 µm) by using phosphate buffer (0.03M, pH 7.2) with flow rate of 2 mL/min which was shown highest activity recovery (82.62%) and purification fold (126.65 times). It was also verified that there was a single band presence and the molecular weight of LP was approximately 85 kDa by SDS-PAGE analysis. Therefore, it is suggested that combination of salting-out and GigaCap S-650M strong cation exchange chromatography could be an efficient and practical method to purify bovine LP.

Purification of lactoperoxidase from bovine milk by integrating the technique of salting-out extraction with cation exchange chromatographic separation

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Purification of lactoperoxidase from bovine milk by integrating the technique of salting-out extraction with cation exchange chromatographic separation