The invention discloses an intervertebral space mutilation system for chunk total nephroureterctomy of thoracolumbar tumors. The intervertebral space mutilation system comprises a protecting body andan inserting strip, wherein the protecting body comprises a head end, a tail end and a body part, wherein the end of the head end is a first concave structure comforming to intervertebral space protrusions; the protecting body adopts a curved structure, and cooperates with thoracolumbar dissection radian; the section of the body part adopts a second concave structure conforming to the thoracolumbar protrusions; a line groove is axially formed in the protecting body along the protecting body; the line groove is used for allowing a fretsaw to be accommodated; two corresponding clamping grooves are formed in the axial direction of the protecting body on two side walls of the line groove; the inserting strip cooperates with the clamping groove; a cavity communicating with the line groove is formed above the head end; and a hook is arranged in the cavity. The intervertebral space mutilation system protected by the invention is simple to operate, safe to use and good in effects.

Intervertebral space mutilation system for chunk total nephroureterctomy of thoracolumbar tumors

The utility model discloses a wire saw guide plate structure for craniotomy. Guide plate, buffering heads are installed on the two sides of the outer portion of the guide plate. An air cushion is attached to the outer part of the buffer head; a placing groove is formed in the guide plate; a clamping ring is mounted in the placement groove; a clamping ring is connected into the clamping ring in a sleeving manner; a protective cover is mounted at the top end of the placement groove; the protective cover is in sliding connection with the guide plate through a sliding groove; the device is scientific and reasonable in structure; a buffer head and an air cushion are arranged; the head end of the guide plate is sharp; the secondary injury to the patient is easily caused by penetrating through the dura mater. A clamping ring and a clamping ring are arranged; according to the guide plate, the installation firmness of the wire saw during guide of the guide plate is improved, the situation thatthe position of the wire saw deviates easily, and the operation quality is affected is avoided, the problems that the wire saw rubs with skull easily during guide, and guide is difficult are solved, meanwhile, after guide is finished, the protective cover can be taken down conveniently, and use is convenient and fast.

Wire saw guide plate structure for craniotomy

The invention discloses human glioma primary cells and an in-vitro isolation and culture method and an application thereof. The cells are named as a low-grade human glioma primary cell XHLG-07 and a high-grade human glioma primary cell XHHG-02 and have the preservation numbers of CCTCC NO:C2018256 and CCTCC NO:C2018215 respectively. The isolation and culture method comprises the steps: acquiring aglioma tissue excised after operation in clinic in vitro, removing blood vessels in the tissue and an adipose tissue by anatomical tweezers, digesting by collagenase/pancreatin, filtering, carrying out low-speed centrifugation to obtain a cell precipitate, carrying out lysis of erythrocytes with an erythrocyte lysis solution, resuspending cells in a DF31 complete culture medium and carrying out inoculation culture. The isolation method can be used for acquiring WHO glioma primary cells with different grades. The obtained cells can be used for analyzing pathogenesis, drug sensitivity and metastasis of human brain gliomas with different grades in vitro and in vivo, and provide research materials more similar to the biological characteristics of clinical tumors for the study of human brain gliomas with different grades.

Human glioma primary cells and in-vitro isolation and culture method and application thereof

The invention discloses a method for building a fluorescent tumor model in nude mice based on primary cells of human cerebral glioma, and belongs to the field of animal models in the medical fields. The method comprises the following steps: S1, culturing of steady-transferring cells: carrying out cell culturing by adopting an improved DMEM/F12 complete culturing medium, and massively amplifying the primary cells capable of stably expressing luciferase; S2, preparation of cell suspensions: repeatedly cleaning the cells by using the improved DMEM/F12 complete culturing medium, collecting the cell suspensions for subsequent use to ensure that the concentration of the cell suspensions is 6*10 cells/mL, and using the suspensions in 40 minutes after the suspensions are prepared; and S3, building of a glioma model of the nude mice: injecting the suspensions in craniums of the nude mice in situ to obtain the mice model capable of stably expressing the luciferase. The fluorescent tumor modelin nude mice based on the human cerebral glioma prepared by adopting the method has the advantages that tumor growth and pathological characteristics of the nude mice are similar to the human cerebral glioma, and growth of the tumors can be observed in real time, so that a desired research model is provided for researching glioma based on in-situ transplanting and tracing of the human cerebral glioma and researching micro-environments of the tumors.

Method for building fluorescent tumor model in nude mice based on primary cells of human cerebral glioma

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Papers

Wire saw guide plate structure for craniotomy

Human glioma primary cells and in-vitro isolation and culture method and application thereof

Method for building fluorescent tumor model in nude mice based on primary cells of human cerebral glioma