The invention discloses a fluorescence labeling composite amplification kit for human Y chromosome 27 STR gene loci, which can simultaneously amplify 27 Y chromosome STR gene loci (including 24 low-mutation-rate Y chromosome STR gene loci and 3 high-mutation-rate Y chromosome STR gene loci) in a single multiplex reaction. By designing the unique gene locus configuration mode and specific primer sequences, the 27 gene loci are divided into 4 groups which are used for different fluorescence labelings. The composite amplification system has the advantages of wide temperature resistance range, high adaptability of the detection material, and high identification capacity for irrelevant and close relative male individuals.

Fluorescence labeling composite amplification kit for human Y chromosome 27 STR gene loci

The invention discloses a new short nucleotide tandem repeat site and application thereof. A short tandem repeat locus G10S0001 has a sequence shown as SEQ ID NO.1, and can be used for preparation of (a) reagents or kits for genetic relationship analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity testing or blood relationship analysis; (d) reagents or kits for detecting whether maternal blood contamination exists in extracted amniotic fluid; and/or (e) kits for detecting whether leucocytes in a bone marrow transplantation receptor are substituted by donor cells. The short tandem repeat locus G10S0001 has high discrimination, and can effectively analyze the genetic relationship.

New short nucleotide tandem repeat site and application thereof

Provided is a multiplex amplification kit for thirty-four loci of human genome DNA, wherein the thirty-four STR loci are: DYS456, DYS458, DYS437, DYS439, DYS392, DYS385a/b, DYS393, DYS391, DYS390, DYS635, DYS438, DYS389I, DYS19, GATA-H4, DYS389II, DYS448, TPOX, D3S1358, D5S818, FGA, CSF1PO, D8S1179, D7S820, TH01, VWA, D13S317, D16S539, D18S51, D21S11, Y-indel, D2S1338, D19S433 and Amelogenin.

Multiplex amplification kit for thirty-four loci of human genome dna

The invention discloses a new short nucleotide tandem repeat sequence locus and application thereof. The invention provides the short tandem repeat locus G2S0002, the sequence of which is shown as SEQ ID NO.:1, and the short tandem repeat locus G2S0002 can be used for preparation of: (a) reagents or kits for genetic relationship analysis; (b) reagents or kits for individual identification; (c) reagents or kits for paternity test or blood relationship analysis; (d) reagents or kits for detecting whether maternal blood contamination exists in extracted amniotic fluid; and/or (e) kits for detecting whether white blood cells in a bone marrow transplantation receptor is replaced by donor cells. The short tandem repeat locus G2S0002 has a high degree of distinction, and can effectively analyze the genetic relationship.

New short nucleotide tandem repeat sequence locus and application thereof

The invention discloses a DNA examination reagent for sample examination, and a special primer thereof. A DNA examination primer group provided by the invention is composed of a primer pair group used for amplifying 21 Y-STR sites, a primer pair for amplifying a sex site Amelogenin, and a primer pair for amplifying an autosome SRT site. Experiments prove that the DNA examination reagent can simultaneously examine the 21 Y-STR sites and the autosome SRT site, and the primer specificity is high, so complete SRT typing can be obtained, peak patterns are sharp and intelligible, the balance is good, no Pull-up peaks or stutter bands appear, no non-specific artificial products appear, and legal medical experts' DNA large scale sample examination requirements are completely met.

DNA examination reagent for sample examination, and special primer thereof

Y-STR locus fluorescent label multiplex amplification system and application thereof

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Y-STR locus fluorescent label multiplex amplification system and application thereof